基于CdSe量子点和Au纳米粒子的毛细管电泳电化学分析研究
发布时间：2018-09-13 06:47
【摘要】：本文研究了基于CdSe量子点和Au纳米粒子的毛细管电泳电化学分析研究。首先研究了CdSe量子点和CdSe/ZnS核壳结构量子点的合成方法,成功合成且进行了一系列表征以及性能比较;将合成的CdSe量子点和CdSe/ZnS核壳结构量子点与毛细管电泳进行联用,构建出毛细管电泳电化学发光平台,检测了不同种类生物胺分子对量子点的增强作用,并同时对其进行分离检测;同时分析分离检测了莱克多巴胺和克仑特罗,并对实际样品进行检测,得到了较好的效果。本文还探讨了基于纳米金辅助信号生成顺序堆积的毛细管电泳免疫分析研究。实验中利用纳米金(AuNPs)作为载体,将辣根过氧化物酶(HRP)和毒素抗体(Ab)同时固定到纳米金(Au NPs)表面,该方法通过纳米金辅助信号生成和顺序堆积在线富集技术实现了对扇贝样品中BTX-B的快速灵敏检测。全文共分为五章:第一章系统介绍了毛细管电泳(CE)的发展历程和应用前景,以及它的基本理论、进样方法、富集技术等;介绍了电化学发光(ECL)的基本原理、电化学发光体系、反应类型、特点等;详细介绍了量子点特点、发光原理、电化学发光方式以及合成方法和应用等等;简要介绍了纳米粒子的有关概念及应用,包括金纳米粒子的特点及应用。第二章探讨了CdSe量子点和CdSe/ZnS核壳结构量子点的合成方法,并对实验中合成的两种量子点进行表征以及性能分析。第三章主要运用合成的CdSe量子点与毛细管电泳联用,构建出一种毛细管电泳量子点电化学发光分析平台,并对胺类物质进行检测。我们对实验条件进行优化,具体包括了实验所用缓冲溶液的种类,pH,浓度,以及分离电压、检测电位,进样时间等。并且在最优条件下,利用构建的电化学分析平台进行胺类物质混合样品的检测。第四章运用合成的CdSe/ZnS核壳结构量子点与毛细管电泳相结合,建成一种毛细管电泳量子点电化学发光平台。我们继续探索了实验影响因素,包括实验所用缓冲溶液的种类,pH,浓度,以及分离电压、检测电位,进样时间等,并在最优条件下,对莱克多巴胺和克伦特罗进行分析分离检测。第五章介绍了一种海生毒素短裸甲藻毒素(BTX)的污染现状及传统检测方法,并且探索了一种纳米材料与毛细管电泳相结合的新型检测方式;进行了一系列实验条件的优化,找出最优条件;在最优条件下测定了贝类样品中的短裸甲藻毒素(BTX)。
[Abstract]:The electrochemical analysis of capillary electrophoresis based on CdSe quantum dots and Au nanoparticles was studied in this paper. Firstly, the synthesis method of CdSe quantum dots and CdSe/ZnS core-shell quantum dots was studied, and a series of characterization and performance comparison were carried out successfully. The synthesized CdSe quantum dots and CdSe/ZnS core-shell structure quantum dots were combined with capillary electrophoresis. A capillary electrophoretic electroluminescent platform was constructed to detect the enhancement of quantum dots by different kinds of biogenic amine molecules and the separation and detection of ractopamine and clenbuterol. And the actual samples were detected and good results were obtained. The immunoassay of capillary electrophoresis based on the stacking of nanocrystalline gold-assisted signals was also discussed in this paper. In the experiment, (AuNPs) was used as carrier to immobilize horseradish peroxidase (HRP) and toxin antibody (Ab) to the surface of nano-gold (Au NPs) at the same time. The rapid and sensitive detection of BTX-B in scallop samples was realized by means of nanocrystalline gold assisted signal generation and sequential stacking on-line enrichment. The thesis is divided into five chapters: the first chapter systematically introduces the development course and application prospect of capillary electrophoresis (CE), its basic theory, sample injection method, enrichment technology, and introduces the basic principle of electroluminescent (ECL), electroluminescent system, and so on. The characteristics of quantum dots, luminescence principle, electrochemiluminescence methods, synthesis methods and applications are introduced in detail. The concepts and applications of nanoparticles are briefly introduced, including the characteristics and applications of gold nanoparticles. In chapter 2, the synthesis methods of CdSe quantum dots and CdSe/ZnS core-shell quantum dots are discussed. In chapter 3, a platform for electrochemiluminescence analysis of quantum dots in capillary electrophoresis was constructed by combining the synthesized CdSe quantum dots with capillary electrophoresis, and the amines were detected. We optimized the experimental conditions, including the pH, concentration, separation voltage, detection potential and injection time of the buffer solution used in the experiment. And under the optimal conditions, the electrochemical analysis platform was used to detect the mixed amines. In chapter 4, we combine the synthesized CdSe/ZnS core-shell quantum dots with capillary electrophoresis to construct a kind of electrochemiluminescence platform for capillary electrophoresis quantum dots. We continue to explore the experimental factors, including pH, concentration, separation voltage, detection potential and injection time of the buffer solution used in the experiment. Under the optimal conditions, the ractopamine and clenbuterol were analyzed and detected. In chapter 5, the pollution status and traditional detection methods of marine toxin (BTX) are introduced, and a new detection method combining nano-material with capillary electrophoresis is explored, and a series of experimental conditions are optimized. The optimum conditions were found and the (BTX). In shellfish samples was determined under the optimum conditions.
【学位授予单位】：青岛科技大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TB383.1;O657.1
[Abstract]:The electrochemical analysis of capillary electrophoresis based on CdSe quantum dots and Au nanoparticles was studied in this paper. Firstly, the synthesis method of CdSe quantum dots and CdSe/ZnS core-shell quantum dots was studied, and a series of characterization and performance comparison were carried out successfully. The synthesized CdSe quantum dots and CdSe/ZnS core-shell structure quantum dots were combined with capillary electrophoresis. A capillary electrophoretic electroluminescent platform was constructed to detect the enhancement of quantum dots by different kinds of biogenic amine molecules and the separation and detection of ractopamine and clenbuterol. And the actual samples were detected and good results were obtained. The immunoassay of capillary electrophoresis based on the stacking of nanocrystalline gold-assisted signals was also discussed in this paper. In the experiment, (AuNPs) was used as carrier to immobilize horseradish peroxidase (HRP) and toxin antibody (Ab) to the surface of nano-gold (Au NPs) at the same time. The rapid and sensitive detection of BTX-B in scallop samples was realized by means of nanocrystalline gold assisted signal generation and sequential stacking on-line enrichment. The thesis is divided into five chapters: the first chapter systematically introduces the development course and application prospect of capillary electrophoresis (CE), its basic theory, sample injection method, enrichment technology, and introduces the basic principle of electroluminescent (ECL), electroluminescent system, and so on. The characteristics of quantum dots, luminescence principle, electrochemiluminescence methods, synthesis methods and applications are introduced in detail. The concepts and applications of nanoparticles are briefly introduced, including the characteristics and applications of gold nanoparticles. In chapter 2, the synthesis methods of CdSe quantum dots and CdSe/ZnS core-shell quantum dots are discussed. In chapter 3, a platform for electrochemiluminescence analysis of quantum dots in capillary electrophoresis was constructed by combining the synthesized CdSe quantum dots with capillary electrophoresis, and the amines were detected. We optimized the experimental conditions, including the pH, concentration, separation voltage, detection potential and injection time of the buffer solution used in the experiment. And under the optimal conditions, the electrochemical analysis platform was used to detect the mixed amines. In chapter 4, we combine the synthesized CdSe/ZnS core-shell quantum dots with capillary electrophoresis to construct a kind of electrochemiluminescence platform for capillary electrophoresis quantum dots. We continue to explore the experimental factors, including pH, concentration, separation voltage, detection potential and injection time of the buffer solution used in the experiment. Under the optimal conditions, the ractopamine and clenbuterol were analyzed and detected. In chapter 5, the pollution status and traditional detection methods of marine toxin (BTX) are introduced, and a new detection method combining nano-material with capillary electrophoresis is explored, and a series of experimental conditions are optimized. The optimum conditions were found and the (BTX). In shellfish samples was determined under the optimum conditions.
【学位授予单位】：青岛科技大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TB383.1;O657.1
【参考文献】
中国期刊全文数据库 前10条
1 冯蕾;赵心怡;赵凤生;;无需两性电解质的小分子等电聚焦微量制备方法[J];化学与生物工程;2015年04期
2 郭颖;李午戊;郑敏燕;黄怡;;石墨烯量子点功能化金纳米粒子的制备及作为过氧化物模拟酶用于葡萄糖检测[J];化学学报;2014年06期
3 马晓年;;毛细管区带电泳法同时测定茶叶中5种活性成分的含量[J];理化检验(化学分册);2013年09期
4 陈珍;黄福荣;;高效毛细管电泳在现代中药中的应用[J];科技视界;2013年22期
5 张召香;张飞;刘营;;基于场放大进样及Au纳米粒子双重富集用于大肠杆菌检测的毛细管电泳电化学免疫分析法[J];化学学报;2012年21期
6 王青;朱红志;羊小海;王柯敏;杨丽娟;丁静;;纳米金颗粒增强信号的表面等离子体共振生物传感器用于甲氧檗因高灵敏检测的研究[J];化学学报;2012年13期
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