稀土掺杂锌基纳米材料的制备及荧光标记性能

发布时间：2018-11-09 11:43

【摘要】：锌基纳米材料所具有的无毒性及优异的生物兼容性使其在生物医学和纳米科技的交叉领域中应用广泛。本文主要通过向生物功能化的锌基发光纳米晶中引入稀土离子掺杂来提高其发光性能,并围绕稀土掺杂锌基纳米材料的发光特性及荧光标记性能研究等问题展开一系列分析研究,主要内容如下:(1)获得了以不同锌基纳米晶作为发光基质的Ce~(3+)掺杂锌基纳米材料,发现立方相的Zn S:Ce纳米晶有相比于六方相的ZnO:Ce纳米晶受到能量激发后电偶极跃迁几率更大,拥有更强的Ce~(3+)离子的特征发光。(2)利用水相合成法制备了发光优异的Eu~(3+)掺杂锌基纳米晶,分析并讨论了ZnS:Eu纳米晶在532 nm激发波长激发下的发光情况,分别在579 nm、595 nm、617 nm、650 nm和714 nm处观察到Eu~(3+)离子的特征发射,其中位于617 nm处~5D_0→~7F_2电偶极跃迁发射最强。(3)选择发光最优的Eu~(3+)掺杂ZnS纳米晶与羊抗人IgG共价结合,以Eu~(3+)掺杂锌基纳米晶的光致发光谱作为探针信号实现了对人IgG的特异性检测,检测限为10nM。
[Abstract]:Zinc based nanomaterials are widely used in biomedicine and nanotechnology because of their nontoxicity and excellent biocompatibility. In this paper, the luminescence properties of zinc-based luminescent nanocrystals were improved by introducing rare earth ions into biologically functionalized zinc-based luminescent nanocrystals. A series of research on luminescence characteristics and fluorescent labeling properties of rare earth doped zinc-based nanomaterials were also carried out. The main contents are as follows: (1) Ce~ _ (3) doped zinc-based nanomaterials with different zinc-based nanocrystals as luminescent substrates were obtained. Compared with hexagonal ZnO:Ce nanocrystals, the cubic Zn S:Ce nanocrystals have a higher electric dipole transition probability than that of hexagonal ZnO:Ce nanocrystals excited by energy. The characteristic luminescence of Ce~ (3) ions is stronger. (2) Eu~ (3) doped zinc-based nanocrystals with excellent luminescence have been prepared by aqueous phase synthesis. The luminescence of ZnS:Eu nanocrystals excited at 532 nm excitation wavelength is analyzed and discussed. The characteristic emission of Eu~ (3) ions was observed at 579 nm,595 nm,617 nm,650 nm and 714 nm, respectively. Among them, ~ 5D_0 ~ 7F_2 dipole transition emission is the strongest at 617 nm. (3) Eu~ (3) -doped ZnS nanocrystals are covalently bound to goat anti-human IgG. Eu~ (3) doped zinc-based nanocrystals were used as probe signals to detect the specificity of human IgG with a detection limit of 10 nm.
【学位授予单位】：长春理工大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TB383.1;TQ422

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