基于复合纳米材料的抗生素电化学适配体传感器的研究
发布时间：2018-11-10 17:57
【摘要】：本文详细介绍了食品中抗生素的分类,来源以及危害。核酸适配体的功能及特点,以及其应用。复合纳米材料的特点和在电化学适配体传感器中的应用。并对电化学适配体传感器做了相关改进,用于检测抗生素。本文对以下内容做了研究:1、基于氧化石墨烯-聚苯胺(GO-PANI)和辣根过氧化物酶(HRP)的信号扩增方案构建了竞争型适配体传感器超灵敏检测土霉素(OTC)。在此方案中,GO-PANI膜修饰在电极表面,金纳米材料(AuNPs)通过恒电位溶出技术电沉积在GO-PANI膜表面。作为捕获探针同时对于OTC具有强亲和力和强选择性的核酸适配体用HRP进行标记。该免疫传感器对于OTC展示了良好的线性响应,其线性范围是4.0×10-6 mg L-1-1.0mg L-1,最低检测限达到2.3×10-6 mg L-1。该传感器具有重复性好,稳定性强的优点,并在实际样品检验中展示出低背景干扰和高灵敏度的优点。2、利用了还原石墨烯-聚苯胺(GR-PANI)和聚酰胺树枝形金纳米复合材料(PAMAM-Au)在电极表面的自组装。这两种材料展现了优秀的电子传导能力和对生物分子的固定能力。在夹心结构的特异性识别基础上,检测卡那霉素的信号是依靠辣根过氧化酶(HRP)催化下,对双氧水(H2O2)电化学还原实现的。上述电化学传感器的线性范围是5×10-6-4×10-2μg m L-1,检测限是4.6×10-6μg m L-1,展示了宽的线性范围。另外,本传感器具有经济性、高选择性和特异性的优势,在实际样品和临床诊断中可以作为检测卡那霉素的一个有用工具。3、利用石墨烯-立体纳米金修饰的玻碳电极(GR-3D Au/GCE)和核酸适配体以及辣根过氧化物酶共同标记的金纳米复合材料(Aptamer-AuNPs-HRP)作为放大策略,构建了新的夹心型电化学传感器。方案中,GR-3D Au通过快速的一步电化学还原得到,具有增强电极表面电子转移速率和固定大量生物分子的优点。Aptamer-AuNPs-HRP对土霉素(OTC)具有强识别作用和强特异性,一个金纳米粒子表面可以固定多个aptamer和HRP,从而提高与OTC的识别作用力以及HRP的催化能力。基于上述信号放大的策略构建的电化学适配体传感器对OTC的线性检测范围是5×10-13-2×10-6 g m L-1,最低检测限是4.98×10-13 g mL-1。另外,该适配体传感器具有高选择性,强特异性的优点,并在实际样品检测中有良好的回收率。
[Abstract]:The classification, source and harm of antibiotics in food were introduced in detail. The function and characteristics of aptamer and its application. Characteristics of composite nanomaterials and their applications in electrochemical adaptor sensors. The electrochemical aptamer sensor was improved to detect antibiotics. In this paper, the following contents were studied: 1. Based on the signal amplification scheme of graphene oxide Polyaniline (GO-PANI) and horseradish peroxidase (HRP), a competitive aptamer sensor for the detection of oxytetracycline (OTC).) was constructed. In this scheme, the GO-PANI film was modified on the electrode surface, and the gold nanocrystalline (AuNPs) was electrodeposited on the surface of the GO-PANI film by potentiostatic stripping technique. Nucleic acid aptamers with strong affinity and selectivity to OTC were labeled with HRP as a capture probe. The immunosensor showed a good linear response to OTC. The linear range was 4.0 脳 10-6 mg L-1-1.0mg L-1 and the minimum detection limit was 2.3 脳 10-6 mg L-1. The sensor has the advantages of good repeatability and high stability, and shows the advantages of low background interference and high sensitivity in the actual sample test. Self-assembly of reduced graphene Polyaniline (GR-PANI) and polyamide dendritic gold nanocomposites (PAMAM-Au) on the electrode surface was carried out. These two materials demonstrate excellent electronic conductivity and immobilization of biomolecules. On the basis of specific recognition of sandwich structure, the signal of kanamycin was determined by electrochemical reduction of hydrogen peroxide (H2O2) catalyzed by horseradish peroxidase (HRP). The linear range of the electrochemical sensor is 5 脳 10-6-4 脳 10-2 渭 g mL -1 and the detection limit is 4.6 脳 10-6 渭 g mL -1, which shows a wide linear range. In addition, the sensor has the advantages of economy, high selectivity and specificity. It can be used as a useful tool for the detection of kanamycin in actual samples and clinical diagnosis. The glassy carbon electrode (GR-3D Au/GCE) and the aptamer of nucleic acid and the gold nanocomposite material (Aptamer-AuNPs-HRP) labeled by horseradish peroxidase (horseradish peroxidase) were used as amplification strategy. A new sandwich electrochemical sensor was constructed. In the scheme, GR-3D Au was obtained by rapid one-step electrochemical reduction, which has the advantages of enhancing the electron transfer rate on the electrode surface and fixing a large number of biomolecules. Aptamer-AuNPs-HRP has strong recognition and specificity to oxytetracycline (OTC). Multiple aptamer and HRP, can be immobilized on the surface of gold nanoparticles to improve the recognition force with OTC and the catalytic ability of HRP. The linear detection range of electrochemical aptamer sensor based on the above signal amplification strategy for OTC is 5 脳 10-13 -2 脳 10-6 g mL -1 and the minimum detection limit is 4.98 脳 10 -13 g mL-1.. In addition, the aptamer sensor has the advantages of high selectivity and strong specificity, and has a good recovery rate in the actual sample detection.
【学位授予单位】：济南大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：O657.1;TB383.1
本文编号：2323181
[Abstract]:The classification, source and harm of antibiotics in food were introduced in detail. The function and characteristics of aptamer and its application. Characteristics of composite nanomaterials and their applications in electrochemical adaptor sensors. The electrochemical aptamer sensor was improved to detect antibiotics. In this paper, the following contents were studied: 1. Based on the signal amplification scheme of graphene oxide Polyaniline (GO-PANI) and horseradish peroxidase (HRP), a competitive aptamer sensor for the detection of oxytetracycline (OTC).) was constructed. In this scheme, the GO-PANI film was modified on the electrode surface, and the gold nanocrystalline (AuNPs) was electrodeposited on the surface of the GO-PANI film by potentiostatic stripping technique. Nucleic acid aptamers with strong affinity and selectivity to OTC were labeled with HRP as a capture probe. The immunosensor showed a good linear response to OTC. The linear range was 4.0 脳 10-6 mg L-1-1.0mg L-1 and the minimum detection limit was 2.3 脳 10-6 mg L-1. The sensor has the advantages of good repeatability and high stability, and shows the advantages of low background interference and high sensitivity in the actual sample test. Self-assembly of reduced graphene Polyaniline (GR-PANI) and polyamide dendritic gold nanocomposites (PAMAM-Au) on the electrode surface was carried out. These two materials demonstrate excellent electronic conductivity and immobilization of biomolecules. On the basis of specific recognition of sandwich structure, the signal of kanamycin was determined by electrochemical reduction of hydrogen peroxide (H2O2) catalyzed by horseradish peroxidase (HRP). The linear range of the electrochemical sensor is 5 脳 10-6-4 脳 10-2 渭 g mL -1 and the detection limit is 4.6 脳 10-6 渭 g mL -1, which shows a wide linear range. In addition, the sensor has the advantages of economy, high selectivity and specificity. It can be used as a useful tool for the detection of kanamycin in actual samples and clinical diagnosis. The glassy carbon electrode (GR-3D Au/GCE) and the aptamer of nucleic acid and the gold nanocomposite material (Aptamer-AuNPs-HRP) labeled by horseradish peroxidase (horseradish peroxidase) were used as amplification strategy. A new sandwich electrochemical sensor was constructed. In the scheme, GR-3D Au was obtained by rapid one-step electrochemical reduction, which has the advantages of enhancing the electron transfer rate on the electrode surface and fixing a large number of biomolecules. Aptamer-AuNPs-HRP has strong recognition and specificity to oxytetracycline (OTC). Multiple aptamer and HRP, can be immobilized on the surface of gold nanoparticles to improve the recognition force with OTC and the catalytic ability of HRP. The linear detection range of electrochemical aptamer sensor based on the above signal amplification strategy for OTC is 5 脳 10-13 -2 脳 10-6 g mL -1 and the minimum detection limit is 4.98 脳 10 -13 g mL-1.. In addition, the aptamer sensor has the advantages of high selectivity and strong specificity, and has a good recovery rate in the actual sample detection.
【学位授予单位】：济南大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：O657.1;TB383.1
【参考文献】
相关期刊论文 前1条
1 张爱春;周存;;DNA电化学生物传感器的研究进展[J];天津工业大学学报;2010年03期
，本文编号：2323181
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