手性离子液体修饰纳米金粒子用于酪氨酸对映体的手性识别

发布时间：2018-11-17 20:09

【摘要】：本文以手性离子液体1-乙基-3-甲基咪唑L-酒石酸盐(EMIML-Tar)为修饰剂合成了EMIML-Tar修饰的金纳米粒子(EMIML-Tar-AuNPs)。采用透射电镜、紫外-可见分光光度计对EMIML-Tar-AuNPs进行了表征。合成的EMIML-Tar-AuNPs平均粒径约为6.5nm,其紫外-可见吸收光谱的最大吸收波长为520nm。进一步将EMIML-Tar-AuNPs用于酪氨酸(Tyr)对映体的手性识别,观察添加不同浓度D-Tyr和L-Tyr的EMIML-Tar-AuNPs的颜色变化及其紫外-可见吸收光谱。结果表明,经过D-Tyr和L-Tyr处理的EMIML-Tar-AuNPs胶体的颜色不同,而且紫外-可见光谱图也存在明显差异。对各浓度光谱图在650nm和520nm处的吸光度比值进行比较,结果发现各浓度D-Tyr的吸光度比值均较低且差别不大,而L-Tyr的吸光度比值均较高,且随着浓度的增大会出现大幅度增加。
[Abstract]:In this paper, EMIML-Tar modified gold nanoparticles (EMIML-Tar-AuNPs) were synthesized with chiral ionic liquid 1-ethyl-3-methyl imidazolium L-tartrate (EMIML-Tar) as modifier. EMIML-Tar-AuNPs was characterized by transmission electron microscope (TEM) and UV-vis spectrophotometer. The average diameter of synthesized EMIML-Tar-AuNPs is about 6.5 nm, and the maximum absorption wavelength of UV-Vis absorption spectrum is 520 nm. EMIML-Tar-AuNPs was further applied to the chiral recognition of tyrosine (Tyr) enantiomers. The color changes and UV-Vis absorption spectra of EMIML-Tar-AuNPs with different concentrations of D-Tyr and L-Tyr were observed. The results show that the color of EMIML-Tar-AuNPs colloid treated by D-Tyr and L-Tyr is different, and the UV-Vis spectrum is also different. The ratio of absorbance of each concentration spectrum at 650nm and 520nm was compared. The results showed that the absorbance ratio of D-Tyr at each concentration was lower and the difference was not significant, but the absorbance ratio of L-Tyr was higher. And with the increase of concentration, there will be a large increase.
【作者单位】：闽江学院化学与化学工程系;
【基金】：国家自然科学基金(No.21405075) 福建省中青年教师教育科研项目(No.JA14248) 闽江学院科技项目(No.MYK14002) 福建省大学生创新创业训练计划项目(No.201410395042)
【分类号】：O657.3;TB383.1

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