荧光-磁共振双模式多功能纳米生物成像探针的制备及应用
发布时间:2018-11-25 09:56
【摘要】:为了开发具有荧光-磁共振双模式成像功能的生物成像探针,有效结合磁共振和荧光2种成像技术的优点,本论文以具有良好单分散性和水溶性的超顺磁性钴掺杂的氧化铁纳米粒子Co_(0.9)Fe_(2.1)O_4为前驱体,将铕(Ⅲ)与4′-苯基-2,2′:6′,2″-联三吡啶-6,6″-二甲胺四乙酸的荧光配合物(PTTA-Eu~(3+))及癌细胞靶向识别分子叶酸(FA)与纳米粒子共价偶联,制备出了1种具有长寿命荧光、超顺磁性(横向弛豫时间T2加权磁共振成像功能)及癌细胞靶向识别性能的多功能纳米生物探针PTTA-Eu~(3+)-CoFeO-FA。这种纳米生物探针不仅可发出铕(Ⅲ)配合物特有的长寿命红色荧光信号,还具有良好的T2加权磁共振成像(MRI)造影剂功能(纳米粒子水溶液的质子横向弛豫速率r_2达183.7L/(mmol·s),使其既可用于癌细胞的时间分辨荧光成像测定,也可用于活体动物的磁共振成像检测。利用所制备的新探针成功地实现了小鼠单核巨噬细胞白血病细胞Raw 264.7的时间分辨荧光成像和昆明鼠的活体T_2加权磁共振成像检测。
[Abstract]:In order to develop a biological imaging probe with the function of fluorescence magnetic resonance dual-mode imaging, the advantages of magnetic resonance imaging and fluorescence imaging are effectively combined. In this paper, the superparamagnetic cobalt doped ferric oxide nanoparticles, Co_ (0.9) Fe_ (2.1) O _ 4, which have good monodispersity and water solubility, are used as precursors. A fluorescent complex (PTTA-Eu~ (3) of 2 "-bipyridine-6" -dimethylaminotetraacetic acid (PTTA-Eu~ (3) and its target recognition molecule folate (FA) were covalently coupled with nanoparticles. Super paramagnetism (transverse relaxation time T 2-weighted magnetic resonance imaging) and multifunctional nano-biological probe PTTA-Eu~ _ (3)-CoFeO-FA. for targeting recognition of cancer cells This kind of nanobiological probe can not only emit the long life red fluorescence signal of europium (鈪,
本文编号:2355685
[Abstract]:In order to develop a biological imaging probe with the function of fluorescence magnetic resonance dual-mode imaging, the advantages of magnetic resonance imaging and fluorescence imaging are effectively combined. In this paper, the superparamagnetic cobalt doped ferric oxide nanoparticles, Co_ (0.9) Fe_ (2.1) O _ 4, which have good monodispersity and water solubility, are used as precursors. A fluorescent complex (PTTA-Eu~ (3) of 2 "-bipyridine-6" -dimethylaminotetraacetic acid (PTTA-Eu~ (3) and its target recognition molecule folate (FA) were covalently coupled with nanoparticles. Super paramagnetism (transverse relaxation time T 2-weighted magnetic resonance imaging) and multifunctional nano-biological probe PTTA-Eu~ _ (3)-CoFeO-FA. for targeting recognition of cancer cells This kind of nanobiological probe can not only emit the long life red fluorescence signal of europium (鈪,
本文编号:2355685
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