碳纳米管及卟啉铁整体柱的制备及应用研究

发布时间：2018-12-09 11:46

【摘要】：本文制备了两种不同类型的整体柱。首先,以卟啉铁(Hemin)和十六烯(C16)为单体,三乙二醇二甲基丙烯酸酯(TEGDA)为交联剂,二甲基甲酰胺(DMF)和异丙醇为致孔剂,过氧化苯甲酰(BPO)与N,N-二甲基苯胺(DMA)为引发剂制备了Hemin聚合物整体柱。利用扫描电子显微镜(SEM)、比表面积及孔径分析仪(BET)对整体柱的结构进行表征。与不加Hemin的整体柱相比,Hemin聚合物整体柱具有更好的渗透性和分离能力,可被应用于分离血浆、鸡卵清等复杂生物样品。此外,该整体柱对于小分子化合物也有一定分离能力。其次,通过氧化还原引发制备了多壁碳纳米管(MWCNT)复合整体柱。在实验中,用辛烯(C8)为单体,TEGDA为交联剂,正丙醇与十二醇为致孔剂,BPO和DMA为引发剂,将羟基化的MWCNT以包埋的方式嵌入到整体柱材料中。然后利用SEM、BET和压汞法(MIP)考察了柱材料的形态和结构。最后,将制备的复合整体柱作为高效液相色谱(HPLC)固定相用于样品的分离分析。与不加MWCNT的整体柱相比较,MWCNT复合整体柱改善了小分子化合物的分离性能,提高了柱效且缩短了分离时间。此外,该整体柱对一些复杂的生物样品也进行了分离。
[Abstract]:In this paper, two different types of monolithic columns were prepared. Firstly, using porphyrin iron (Hemin) and hexadecene (C16) as monomers, triethylene glycol dimethacrylate (TEGDA) as crosslinking agent, dimethylformamide (DMF) and isopropanol as pore-forming agents, benzoyl peroxide (BPO) and N, N-dimethylaniline (DMA) was used as initiator to prepare Hemin polymer monolithic column. The structure of the monolithic column was characterized by scanning electron microscope (SEM) (SEM), surface area and pore size analyzer (BET). Compared with the monolithic column without Hemin, the Hemin polymer monolithic column has better permeability and separation ability, and can be applied to the separation of complex biological samples such as plasma, chicken egg albumin and so on. In addition, the monolithic column also has a certain separation ability for small molecular compounds. Secondly, multi-walled carbon nanotubes (MWCNT) composite monolithic columns were prepared by redox initiation. Using octene (C 8) as monomer, TEGDA as crosslinking agent, n-propanol and dodecanol as pore-forming agent and BPO and DMA as initiators, hydroxylated MWCNT was embedded in monolithic column materials. Then the morphology and structure of the column materials were investigated by SEM,BET and (MIP). Finally, the composite monolithic column was used as (HPLC) stationary phase for the separation and analysis of samples. Compared with the monolithic column without MWCNT, the MWCNT composite monolithic column improved the separation performance of small molecular compounds, improved the column efficiency and shortened the separation time. In addition, some complex biological samples were separated by the monolithic column.
【学位授予单位】：河北大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TB383.1;O652.63

【相似文献】