几种纳米材料在光学生物成像中的应用研究

发布时间：2018-12-14 21:28

【摘要】：生物光子学是由先进光子学技术和生物技术交叉结合所形成的一门前沿的学科。在生物光子学的研究中,光学生物成像是其中的一个分支。本论文将几种新型纳米颗粒与先进的光学系统相结合。开展了一系列光学生物成像方面的工作。论文中,我们介绍了近红外荧光染料掺杂的纳米颗粒、上转换纳米颗粒,以及它们的表面修饰方法等,并对它们进行了表征。在生物成像方面,这两种纳米颗粒都可以作为高效的荧光探针。我们将近红外荧光染料掺杂的纳米颗粒应用于小鼠的前哨淋巴结标记和活体功能性成像以及活体深层显微成像。上转换纳米颗粒在生物成像方面也有着很多的优势,我们提出了一种新方法激发上转换纳米颗粒,与传统的激发方式相比,这种激发方式可以获得更大的成像深度。在生物成像领域,新型光学系统的设计与搭建为光学生物成像提供了有效的工具。我们设计并搭建了基于倏逝场激发的全内反射荧光显微系统、显微拉曼探测系统以及多光子荧光显微扫描成像系统等,为后续的光学生物成像研究提供了有利的条件。
[Abstract]:Biophotonics is a frontier subject formed by the combination of advanced photonics and biotechnology. In the study of biological photonics, optical imaging is one of the branches. In this paper, several new nanoparticles are combined with advanced optical systems. A series of work on optical biometric imaging has been carried out. In this paper, the near infrared fluorescent dye doped nanoparticles, up-conversion nanoparticles and their surface modification methods are introduced and characterized. In biological imaging, both nanoparticles can be used as highly efficient fluorescent probes. We used near infrared fluorescent dye doped nanoparticles for sentinel lymph node labeling and in vivo functional imaging and in vivo deep microscopic imaging of mice. The up-conversion nanoparticles also have many advantages in biological imaging. We propose a new method to excite the up-converted nanoparticles. Compared with the traditional excitation method, this excitation method can obtain greater imaging depth. In the field of biological imaging, the design and construction of new optical systems provide an effective tool for optical biometric imaging. We have designed and built a total internal reflection fluorescence microscopy system based on evanescent field excitation, a Raman microprobe system and a multi-photon fluorescence microscopy scanning imaging system, which provide favorable conditions for the further study of optical biological imaging.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TB383.1;O439

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