量子点和纳米金在药物及蛋白分析中的应用

发布时间：2018-12-17 09:46

【摘要】：纳米材料由于具有独特的光电性能,受到众多领域研究者的广泛关注。近年来,基于量子点和纳米金构建光学传感器成为迅速发展的领域之一。本文研究构筑了两种检测药物及蛋白的光学传感器。一种是基于目标检测物能够有效地猝灭或增强巯基乙酸(TGA)修饰的Cd Te量子点的荧光,另一种是基于检测物能够引起纳米金团聚建立的比色分析方法。主要内容如下:(1)分别基于替加环素对Cd Te量子点的荧光猝灭作用和使纳米金团聚的作用建立了两种检测替加环素的新方法,并进行了比较。在优化实验条件下,Cd Te量子点荧光猝灭的程度与替加环素浓度在0.11-35.56μg m L-1范围内呈现良好的线性关系,检出限为3.93×10-2μg m L-1;纳米金比色法测定替加环素的线性范围为5.98×10-3-1.44×10-1μg m L-1和0.30-2.99μg m L-1,检出限为1.07×10-3μg m L-1。将上述两种方法用于实际样品中替加环素的分析,回收率在94.60%-104.44%之间。(2)分别基于盐酸林可霉素(LCM)对Cd Te量子点的荧光增敏作用和使纳米金团聚的性质建立了两种检测LCM的新方法,并进行了比较。在优化条件下,在1-240μg m L-1范围内,LCM浓度与量子点荧光增强程度呈良好的线性关系,检出限为2.63×10-1μg m L-1;在1.00×10-3-2.00×10-2μg m L-1及3.00×10-2-1.20×10-1μg m L-1范围时,LCM浓度与纳米金吸光度比值(A650/A519)分别呈现良好的线性关系,检出限为1.27×10-4μg m L-1。将方法用于LCM片剂的分析,结果满意。(3)分别基于依替米星(ETM)对Cd Te量子点的荧光增敏作用和使纳米金团聚的性质建立了两种检测ETM的新方法,并进行了比较。在优化实验条件下,量子点荧光增敏程度与ETM浓度在5.55-266.40 ng m L-1范围内呈现良好的线性关系,检出限为1.28 ng m L-1;纳米金比色法测定ETM的线性范围为1.00-20.00 ng m L-1及28.00-84.00 ng m L-1,检出限为0.26 ng m L-1。两种方法用于实际样品中ETM的测定,回收率在95.0%-104.9%之间。(4)利用荧光光谱法、紫外光谱法、同步荧光及三维荧光光谱法研究了TGA-Cd Te量子点与FTO蛋白的相互作用。结果表明,TGA-Cd Te量子点能够猝灭FTO蛋白的荧光,猝灭机理为静态猝灭。通过计算得到了TGA-Cd Te量子点与FTO蛋白作用的结合常数及热力学参数等。此外,基于FTO蛋白对TGA-Cd Te量子点的荧光增敏作用,建立了测定FTO蛋白的方法。线性范围为5.52×10-9-6.62×10-7 mol L-1,检出限为1.14×10-9 mol L-1。方法用于合成样品中FTO蛋白的检测,结果满意。
[Abstract]:Nanomaterials have attracted wide attention due to their unique optoelectronic properties. In recent years, the construction of optical sensors based on quantum dots and nanocrystalline gold has become one of the rapidly developing fields. In this paper, two optical sensors for the detection of drugs and proteins have been developed. One is based on the fact that the target detector can effectively quench or enhance the fluorescence of Cd Te quantum dots modified by mercaptoacetic acid (TGA), and the other is a colorimetric analysis method based on the method of colorimetric analysis, which can cause nano-gold agglomeration. The main contents are as follows: (1) based on the fluorescence quenching effect of tegacycline on Cd Te quantum dots and the effect of nano-gold agglomeration, two new methods for the detection of tegacycline were established and compared. Under the optimized experimental conditions, the fluorescence quenching of, Cd Te quantum dots showed a good linear relationship with the concentration of tegicycline in the range of 0.11-35.56 渭 g mL ~ (-1), and the detection limit was 3.93 脳 10 ~ (-2) 渭 g / mL ~ (-1). The linear range of tigicycline was 5.98 脳 10 ~ (-3) -1.44 脳 10 ~ (-1) 渭 g mL ~ (-1) and 0.30-2.99 渭 g / mL ~ (-1) with the detection limit of 1.07 脳 10 ~ (-3) 渭 g / m ~ (-1). The above two methods have been applied to the analysis of tegacycline in actual samples. The recoveries ranged from 94.60% to 104.44%. (2) based on the fluorescence sensitizing effect of lincomycin (LCM) on Cd Te quantum dots and the properties of nano-gold agglomeration, two new methods for the detection of LCM were established and compared. Under the optimized conditions, there was a good linear relationship between the concentration of LCM and the fluorescence enhancement of quantum dots in the range of 1-240 渭 g mL ~ (-1), and the detection limit was 2.63 脳 10 ~ (-1) 渭 g / m ~ (-1). In the range of 1.00 脳 10-3-2.00 脳 10-2 渭 g mL-1 and 3.00 脳 10-2-1.20 脳 10-1 渭 g mL-1, the linear relationship between the concentration of LCM and the gold absorbance ratio (A650/A519) was good. The detection limit is 1.27 脳 10 ~ (-4) 渭 g mL ~ (-1). The method has been applied to the analysis of LCM tablets with satisfactory results. (3) based on the fluorescence sensitizing effect of etemicin (ETM) to Cd Te quantum dots and the properties of nano-gold agglomeration, two new methods for the detection of ETM have been established and compared. Under the optimized experimental conditions, there was a good linear relationship between the fluorescence sensitivity of QDs and the concentration of ETM in the range of 5.55-266.40 ng mL ~ (-1), and the detection limit was 1.28 ng mL ~ (-1). The linear ranges for the determination of ETM by nano-gold colorimetry were 1.00-20.00 ng mL -1 and 28.00-84.00 ng mL -1, and the detection limit was 0.26 ng mL -1. The two methods have been applied to the determination of ETM in practical samples. The recoveries are in the range of 95.0-104.9%. (4) fluorescence spectrometry, UV spectrophotometry, The interaction between TGA-Cd Te quantum dots and FTO protein was studied by synchronous fluorescence and three dimensional fluorescence spectroscopy. The results show that TGA-Cd Te QDs can quench the fluorescence of FTO protein, and the quenching mechanism is static quenching. The binding constants and thermodynamic parameters of the interaction between TGA-Cd Te quantum dots and FTO protein were calculated. In addition, based on the fluorescence sensitizing effect of FTO protein on TGA-Cd Te quantum dots, a method for the determination of FTO protein was established. The linear range is 5.52 脳 10-9-6.62 脳 10-7 mol L-1 and the detection limit is 1.14 脳 10-9 mol L-1. The method has been applied to the determination of FTO protein in synthetic samples with satisfactory results.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TB383.1;TP212

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