FDTD仿真金属纳米颗粒LSPR特性及在生物光子学上的应用研究
发布时间：2018-12-19 11:09
【摘要】：近年来,纳米科学领域的蓬勃发展使得金属纳米颗粒的制备表征取得了瞩目的成就,其具有的一些独特的光学、电学特性也逐渐呈现在世人面前。发展至今,后续的应用研究已经在物理、化学、生命医学等领域得到了广泛的开展。目前,金属纳米颗粒不仅成为了进行光与物质相互作用研究最好的平台,而且为生命科学领域提供了重要的研究方法和检测途径。因此,研究关于金属纳米结构独具魅力的特性及其与生物分子间的相互作用,对于研究开发性能稳定、效果良好的近场成像技术以及进行生物分子空间分辨特性检测等工作的开展具有非常积极而重大的意义。基于金属纳米颗粒在生命科学研究中的重要性,结合本课题组的研究工作基础,本论文以金属纳米颗粒为研究对象,使用时域有限差分法(FDTD)仿真模拟研究了金属纳米颗粒的光学特性,及其在生物光子学领域的应用。通过利用局域表面等离子体激元共振(LSPR)相互作用机制设计了近场LSPR信号放大器,并分析了在生物光子学领域的潜在应用。在金属纳米颗粒与荧光分子相互作用机制的基础上,拓展了金属纳米颗粒在荧光分子空间分辨特性探测上的应用。不仅对金属纳米颗粒的LSPR特性进行了深入的研究讨论,同时对在生命科学领域的应用进行了优化和开拓。本论文主要的研究成果如下：1、系统研究了金属纳米颗粒二聚体的LSPR特性。考察了LSPR耦合相互作用对二聚体金属结构局域电场分布的影响。相比较单颗粒局域电场分布,二聚体纳米结构具有更强的局域电场增强能力。使用金属自由电荷分布及电荷相互作用机制深入分析了二聚体纳米结构的LSPR特性,这对我们接下来金属纳米颗粒二聚体放大器的设计和优化提供了理论指导；2、设计了一种基于二聚体结构的LSPR放大器,实现了在局域范围内的电场放大,这对一些微弱信号的增强探测有着积极的作用。通过在二聚体外部包裹增益材料的方法来克服金属纳米颗粒的吸收损耗,极大的增强了二聚体结构周围的局域电场。研究二聚体纳米粒子间距d的大小对局域电场分布的影响,选择合适的间距情况下考察了LSPR放大器的近场增强能力,探讨了它的应用前景.相关结果使用时域有限差分法(FDTD)计算得到.通过调节二聚体纳米粒子的间距,实现特定的信号增强。3、研究了利用金属纳米颗粒附近分子的荧光发射原理,进行生物分子间的相互作用研究的可行性。拓宽了探测表征手段,除了常用的荧光强度等参数,我们还利用量子效率、荧光寿命等参数来进行相关表征。通过灵活多样的探测手段,此方法可以应用于蛋白、DNA、抗体-抗原相关的结合作用研究。并且可以更加灵敏地进行分子间结合作用研究。使用FDTD仿真计算了处于金属纳米颗粒附近荧光分子的发射特性,探讨了能量交换的机理。通过对比不同条件下处于金属纳米颗粒附近荧光分子的发射特性,根据量子效率和荧光寿命来探测荧光分子的空间分辨特性,从而研究生物分子间的结合作用。
[Abstract]:In recent years, the development of the nano-science field has made the preparation and characterization of the metal nano-particles remarkable achievement, and has some unique optical and electrical characteristics. So far, the follow-up research has been carried out in the fields of physics, chemistry, life medicine and so on. At present, the metal nanoparticles not only become the best platform for the interaction of light and materials, but also provide important research methods and methods for the field of life science. Therefore, the study on the characteristics of the unique charm of the metal nano-structure and its interaction with the biological molecules, the research and development performance is stable, It is of great significance to carry out the near-field imaging technology with good effect and to carry out the detection of the biological molecule space resolution property. Based on the importance of metal nanoparticles in the research of life science, and based on the research work of the research group, the optical properties of the metal nanoparticles were studied by using the time-domain finite difference method (FDTD) simulation. and the application thereof in the field of biological photonics. The near field LSPR signal amplifier is designed by the local surface plasmon resonance (LSPR) interaction mechanism, and the potential application in the field of biological photonics is analyzed. On the basis of the interaction mechanism between the metal nanoparticles and the fluorescent molecules, the application of the metal nanoparticles in the detection of the space-resolved properties of the fluorescent molecules is expanded. Not only the LSPR characteristics of the metal nanoparticles have been studied in depth, but also the application in the field of life science is optimized and developed. The main results of this thesis are as follows: 1. The LSPR characteristic of the metal nanoparticle dimer is studied. The effect of the LSPR coupling interaction on the local electric field distribution of the dimer metal structure was investigated. Compared with the single-particle local electric field distribution, the dimeric nanostructures have a stronger local electric field enhancement capability. The LSPR characteristic of the dimer nano-structure is deeply analyzed by using the metal free charge distribution and the charge interaction mechanism, which provides the theoretical guidance for the design and optimization of the next metal nano-particle dimer amplifier; and 2, an LSPR amplifier based on the dimer structure is designed, The electric field amplification in the local area is realized, which has a positive effect on the enhancement detection of some weak signals. by wrapping the gain material outside the dimer, the absorption loss of the metal nanoparticles is overcome, and the local electric field around the dimer structure is greatly enhanced. The influence of the size of the nano-particle spacing d on the distribution of the local electric field is studied, and the near field enhancement of the LSPR amplifier is investigated under the condition of proper spacing, and its application prospect is discussed. The correlation results are calculated using the time-domain finite difference method (FDTD). By adjusting the spacing of the dimer nanoparticles, the specific signal enhancement was achieved. 3. The fluorescence emission principle of the molecules near the metal nanoparticles was used to study the interaction between the biomolecules. In addition to the commonly used parameters such as fluorescence intensity, we also use the parameters of quantum efficiency, fluorescence lifetime and so on to make relevant representation. The method can be applied to the binding action of protein, DNA and antibody-antigen. and the intermolecular binding effect can be more sensitively carried out. The emission characteristics of the fluorescent molecules in the vicinity of the metal nanoparticles were calculated by the FDTD method, and the mechanism of energy exchange was discussed. by comparing the emission characteristics of the fluorescent molecules in the vicinity of the metal nanoparticles under different conditions, the spatial resolution characteristics of the fluorescent molecules are detected according to the quantum efficiency and the fluorescence lifetime, and the binding action between the biomolecules is studied.
【学位授予单位】：华东师范大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TB383.1;O657.3
[Abstract]:In recent years, the development of the nano-science field has made the preparation and characterization of the metal nano-particles remarkable achievement, and has some unique optical and electrical characteristics. So far, the follow-up research has been carried out in the fields of physics, chemistry, life medicine and so on. At present, the metal nanoparticles not only become the best platform for the interaction of light and materials, but also provide important research methods and methods for the field of life science. Therefore, the study on the characteristics of the unique charm of the metal nano-structure and its interaction with the biological molecules, the research and development performance is stable, It is of great significance to carry out the near-field imaging technology with good effect and to carry out the detection of the biological molecule space resolution property. Based on the importance of metal nanoparticles in the research of life science, and based on the research work of the research group, the optical properties of the metal nanoparticles were studied by using the time-domain finite difference method (FDTD) simulation. and the application thereof in the field of biological photonics. The near field LSPR signal amplifier is designed by the local surface plasmon resonance (LSPR) interaction mechanism, and the potential application in the field of biological photonics is analyzed. On the basis of the interaction mechanism between the metal nanoparticles and the fluorescent molecules, the application of the metal nanoparticles in the detection of the space-resolved properties of the fluorescent molecules is expanded. Not only the LSPR characteristics of the metal nanoparticles have been studied in depth, but also the application in the field of life science is optimized and developed. The main results of this thesis are as follows: 1. The LSPR characteristic of the metal nanoparticle dimer is studied. The effect of the LSPR coupling interaction on the local electric field distribution of the dimer metal structure was investigated. Compared with the single-particle local electric field distribution, the dimeric nanostructures have a stronger local electric field enhancement capability. The LSPR characteristic of the dimer nano-structure is deeply analyzed by using the metal free charge distribution and the charge interaction mechanism, which provides the theoretical guidance for the design and optimization of the next metal nano-particle dimer amplifier; and 2, an LSPR amplifier based on the dimer structure is designed, The electric field amplification in the local area is realized, which has a positive effect on the enhancement detection of some weak signals. by wrapping the gain material outside the dimer, the absorption loss of the metal nanoparticles is overcome, and the local electric field around the dimer structure is greatly enhanced. The influence of the size of the nano-particle spacing d on the distribution of the local electric field is studied, and the near field enhancement of the LSPR amplifier is investigated under the condition of proper spacing, and its application prospect is discussed. The correlation results are calculated using the time-domain finite difference method (FDTD). By adjusting the spacing of the dimer nanoparticles, the specific signal enhancement was achieved. 3. The fluorescence emission principle of the molecules near the metal nanoparticles was used to study the interaction between the biomolecules. In addition to the commonly used parameters such as fluorescence intensity, we also use the parameters of quantum efficiency, fluorescence lifetime and so on to make relevant representation. The method can be applied to the binding action of protein, DNA and antibody-antigen. and the intermolecular binding effect can be more sensitively carried out. The emission characteristics of the fluorescent molecules in the vicinity of the metal nanoparticles were calculated by the FDTD method, and the mechanism of energy exchange was discussed. by comparing the emission characteristics of the fluorescent molecules in the vicinity of the metal nanoparticles under different conditions, the spatial resolution characteristics of the fluorescent molecules are detected according to the quantum efficiency and the fluorescence lifetime, and the binding action between the biomolecules is studied.
【学位授予单位】：华东师范大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TB383.1;O657.3
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1 杜松涛,熊贵光;复合金属纳米颗粒多孔硅的光学非线性特性[J];武汉大学学报(理学版);2003年05期
2 何武强,何宝林,陈益贤,廖小娟,刘汉范;溶剂稳定的钯金属纳米颗粒的制备[J];中南民族大学学报(自然科学版);2004年04期
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