磁性纳米颗粒层层自组装膜对原代小鼠骨髓细胞的生长促进作用（英文）

发布时间：2019-05-07 14:00

【摘要】：磁场一直以来都被认为对骨生长具有促进作用.磁性纳米颗粒可以被看作是一个磁偶极子,因此宏观的磁性纳米颗粒组装膜也可能对附近的物体具有磁效应.本文通过层层自组装方法在聚乳酸支架表面制备了宏观γ-Fe_2O_3纳米颗粒组装膜,研究了γ-Fe_2O_3纳米颗粒组装膜对原代小鼠骨髓细胞的磁作用.原代小鼠骨髓细胞从小鼠体内新鲜提取,并在前述生物材料表面培养.定量PCR用来定量表征细胞效应,磁场的影响通过检测一种刚刚发现的磁感应蛋白来指示.结果表明,表面纳米颗粒组装可以显著增强聚合物支架的力学性质,促进细胞生长和分化.磁感应蛋白检测结果表明这是由于磁性纳米颗粒组装导致的磁效应引起的.本文用磁感应蛋白证明了磁性纳米颗粒层层自组装膜可以通过对细胞的磁效应促进干细胞的生长和分化,该磁性纳米颗粒组装膜将会促进新一代组织工程支架的研发,有可能将物理刺激效应引入到体内局部组织修复中.
[Abstract]:Magnetic fields have long been thought to promote bone growth. Magnetic nanoparticles can be regarded as a magnetic dipole, so macroscopically assembled magnetic nanoparticles may also have a magnetic effect on nearby objects. In this paper, macroscopical 纬-Fe_2O_3 nanoparticles were prepared on the surface of poly (lactic acid) scaffolds by layer-by-layer self-assembly method, and the magnetic effects of 纬-Fe_2O_3 nanoparticles on primary mouse bone marrow cells were studied. Primary mouse bone marrow cells were freshly extracted from mice and cultured on the surface of the above biomaterials. Quantitative PCR is used to quantify cell effects, and magnetic field effects are indicated by the detection of a newly discovered magnetic induction protein. The results showed that surface nano-particles assembly could significantly enhance the mechanical properties of polymer scaffolds and promote cell growth and differentiation. Magnetic induction protein test results show that this is due to magnetic effect caused by the assembly of magnetic nanoparticles. In this paper, we demonstrated that the magnetic nanoparticles layer-by-layer self-assembled membrane can promote the growth and differentiation of stem cells through the magnetic effect on cells. The magnetic nanoparticle-assembled membrane will promote the development of a new generation of tissue engineering scaffolds, and the self-assembly membrane of magnetic nanoparticles can promote the growth and differentiation of stem cells. It is possible to introduce physical stimulation into local tissue repair in vivo.
【作者单位】： School
【基金】：supported by the National Basic Research Program of China(2013CB733801) the National Natural Science Foundation of China(21273002 and 61601227) fund for the top doctoral thesis of Chinese Education Ministry(201174) the Natural Science Foundation of Jiangsu Province(BK20160939 and BK20130608) supports from Collaborative Innovation Center of Suzhou Nano Science and Technology
【分类号】：TB383.1;R318.08

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