无机类纳米材料抗菌性研究以及对多头绒泡菌影响的初探
发布时间：2019-05-15 16:39
【摘要】：本论文主要研究了银纳米颗粒(Ag NPs)和Ag NPs包覆氧化锌纳米颗粒(Zn O NPs)的复合纳米材料(Zn O@Ag NPs)的制备及其抗菌性能和初步研究了三种无机纳米颗粒对多头绒泡菌的影响。具体内容如下:1.以Na BH4为还原剂,Ag NO3为银源,聚乙烯吡咯烷酮(PVP)和羧化壳聚糖(Cchitosan)为分散剂,并在制备过程中加入适量的Na OH,通过调整制备参数制备出了分别以PVP和C-chitosan为分散剂的Ag NPs分散液,二者的形状为球形,晶体结构类型均为FCC,粒径分布范围分别为14.4-36.7 nm和8-38.4 nm,均在水溶液中均匀分散。采用抑菌圈法和连续LB培养基稀释法对上述两种Ag NPs的抗菌性能进行了评价,结果表明本论文制备出的Ag NPs的抗菌性能优异,并对酵母菌的生长也有一定的抑制作用;本论文也借助SEM和激光扫描共聚焦显微镜(CLSM)等技术手段对Ag NPs的抗菌机制进行了探究。Ag NPs的抗菌作用主要是造成细菌或真菌细胞内部大量产生活性氧物质(ROS)引起细菌或真菌细胞的氧化损伤的方式来实现的;2.以Zn O NPs为核心,以Ag(TEA)2+为银源,以甲醛或水合肼为还原剂,采用简单的三步法制备出了Zn O@Ag NPs。结果表明,Zn O@Ag NPs表面的Ag NPs的晶体结构类型为面心立方结构(fcc),形状为球形或椭球形,粒径变化范围为10-42 nm,Zn O@Ag NPs的包覆率也出现相应的变化。采用抑菌圈法和连续LB培养基稀释法对Zn O@Ag NPs的抗菌性能进行了评价。结果表明Zn O@Ag NPs的抗菌性能优异;而且Zn O@Ag NPs对酵母菌的生长也有一定的抑制作用;Zn O@Ag NPs对金黄色葡萄球菌的抗菌性能与PVP分散的Ag NPs比较接近,但是其银的含量仅为后者的25%;其对酵母菌的MIC值为PVP-Ag NPs的1.5倍,为C-chitosan-Ag NPs的3倍,但是其银的含量仅为后两者的25%,因此,其对酵母菌的综合抑制作用也与PVP分散的Ag NPs相当。本论文也借助CLSM对Ag NPs的抗菌机制进行了探究。Zn O@Ag NPs的抗菌作用主要与其造成细菌或真菌细胞内部大量产生活性氧物质(ROS)引起细菌或真菌细胞的氧化损伤有关;3.以多头绒泡菌为作用对象,本论文初步研究了Zn O NPs、二氧化钛纳米颗粒(Ti O2 NPs)和第2章中的PVP分散的Ag NPs的毒性。Zn O NPs、Ti O2 NPs和Ag NPs对多头绒泡菌的明显抑制浓度分别为1750μg/m L、15000μg/m L和300μg/m L,Ag NPs对多头绒泡菌的毒性要明显强于Zn O NPs和Ti O2 NPs,Ti O2 NPs最小。在较高浓度的Zn O NPs作用下,多头绒泡菌出现了 逃逸‖现象,Ti O2 NPs和Ag NPs却没有。在Zn O NPs、Ti O2 NPs和Ag NPs作用下,多头绒泡菌的MDA产生均与纳米颗粒的浓度存在依赖关系,随纳米颗粒的浓度的增大而先增大后减小,说明多头绒泡菌原质团内部的氧化损伤程度越来越大,增大到一定程度时,多头绒泡菌出现死亡,而多头绒泡菌出现死亡也导致MDA产生量下降,这为纳米颗粒的ROS毒性机制进一步提供了证据。
[Abstract]:The preparation and antibacterial properties of the composite nano-materials (Zn-O@Ag NPs) coated with silver nano-particles (Ag-NPs) and Ag-NPs-coated zinc oxide nanoparticles (Zn-NPs) were studied in this paper. The effects of the three kinds of inorganic nanoparticles on the multi-head chorionic bacteria were studied. The specific content is as follows:1. Na-BH4 is used as a reducing agent, and the Ag-NO3 is a silver source, and the polyethylene is used as a dispersing agent, and a proper amount of Na-OH is added in the preparation process, and the Ag NPs dispersion liquid with the PVP and the C-chitosan as a dispersing agent is prepared by adjusting the preparation parameters, The shape of the two is spherical, the crystal structure type is FCC, the particle size distribution range is 14.4-36.7 nm and 8-38.4 nm, respectively, and is uniformly dispersed in the aqueous solution. The antibacterial properties of the above two kinds of Ag NPs were evaluated by the method of bacteriostatic ring and continuous LB medium dilution. The results showed that the antibacterial properties of the Ag NPs prepared by the method were excellent, and the growth of the yeast was also inhibited. The antibacterial mechanism of Ag NPs was also studied by means of SEM and laser scanning confocal microscope (CLSM). The antibacterial effect of Ag NPs is mainly caused by a large amount of reactive oxygen species (ROS) in bacteria or fungal cells causing oxidative damage to bacteria or fungal cells;2. Zn-O@Ag NPs was prepared by using an Ag (TEA)2 + as a silver source and using a simple three-step method as a reducing agent with Zn O NPs as the core. The results show that the crystal structure of the Ag NPs on the surface of the Zn-O@Ag NPs is the surface-centered cubic structure (fcc), the shape is spherical or ellipsoidal, the particle size variation range is 10-42nm, and the cladding rate of the Zn-O@Ag NPs also changes correspondingly. The antibacterial performance of Zn-O@Ag NPs was evaluated by the method of bacteriostatic ring and continuous LB medium dilution. The results show that the antibacterial performance of Zn-O@Ag-NPs is excellent, and the growth of the Zn-O@Ag-NPs has a certain inhibition effect on the growth of the yeast. The antibacterial performance of the Zn-O@Ag-NPs is close to that of the Ag-NPs in which the PVP is dispersed, but the content of the silver is only 25% of the latter; The MIC value of the yeast is 1.5 times that of the PVP-Ag NPs, which is 3 times that of the C-chitosan-Ag NPs, but the content of the silver is only 25% of the latter, so that the comprehensive inhibition effect on the yeast is also equivalent to that of the PVP-dispersed Ag NPs. The antibacterial mechanism of Ag NPs was also studied by CLSM. The antibacterial action of the Zn O@Ag NPs is mainly related to the oxidative damage of bacteria or fungal cells caused by a large amount of reactive oxygen species (ROS) inside the bacterial or fungal cells. In this paper, the toxicity of Zn-O-NPs, titanium dioxide nanoparticles (TiO2 NPs) and PVP-dispersed Ag-NPs in Chapter 2 was studied in this paper. The obvious inhibitory concentration of Zn O NPs, Ti O 2 NPs and Ag NPs on the multi-headed chorionic bacteria was 1750. m u.g/ m L,15000. m u.g/ m L and 300. m u.g/ m L, respectively. The toxicity of Ag NPs to the multi-headed chorionic bacteria was significantly stronger than that of Zn O NPs and Ti O2 NPs, and the minimum of Ti O2 NPs. Under the action of higher concentration of Zn-O NPs, the phenomenon of escape was found in the multi-headed suede, and the NPs and Ag-NPs of Ti-2 were not. Under the action of Zn-O-NPs, Ti-O2 NPs and Ag-NPs, the content of MDA in the multi-headed chorionic bacteria is dependent on the concentration of the nano-particles, and then the concentration of the nano-particles is increased, and then the concentration of the nano-particles is reduced, so that the degree of oxidation damage inside the original mass of the multi-head chorionic bacteria is more and more large, When increasing to a certain extent, the occurrence of the death of the multi-headed suede, and the decrease of the production of the MDA, the occurrence of the death of the multi-headed choriococcus has further provided the evidence for the ROS toxicity mechanism of the nano-particles.
【学位授予单位】：深圳大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TB383.1
本文编号：2477636
[Abstract]:The preparation and antibacterial properties of the composite nano-materials (Zn-O@Ag NPs) coated with silver nano-particles (Ag-NPs) and Ag-NPs-coated zinc oxide nanoparticles (Zn-NPs) were studied in this paper. The effects of the three kinds of inorganic nanoparticles on the multi-head chorionic bacteria were studied. The specific content is as follows:1. Na-BH4 is used as a reducing agent, and the Ag-NO3 is a silver source, and the polyethylene is used as a dispersing agent, and a proper amount of Na-OH is added in the preparation process, and the Ag NPs dispersion liquid with the PVP and the C-chitosan as a dispersing agent is prepared by adjusting the preparation parameters, The shape of the two is spherical, the crystal structure type is FCC, the particle size distribution range is 14.4-36.7 nm and 8-38.4 nm, respectively, and is uniformly dispersed in the aqueous solution. The antibacterial properties of the above two kinds of Ag NPs were evaluated by the method of bacteriostatic ring and continuous LB medium dilution. The results showed that the antibacterial properties of the Ag NPs prepared by the method were excellent, and the growth of the yeast was also inhibited. The antibacterial mechanism of Ag NPs was also studied by means of SEM and laser scanning confocal microscope (CLSM). The antibacterial effect of Ag NPs is mainly caused by a large amount of reactive oxygen species (ROS) in bacteria or fungal cells causing oxidative damage to bacteria or fungal cells;2. Zn-O@Ag NPs was prepared by using an Ag (TEA)2 + as a silver source and using a simple three-step method as a reducing agent with Zn O NPs as the core. The results show that the crystal structure of the Ag NPs on the surface of the Zn-O@Ag NPs is the surface-centered cubic structure (fcc), the shape is spherical or ellipsoidal, the particle size variation range is 10-42nm, and the cladding rate of the Zn-O@Ag NPs also changes correspondingly. The antibacterial performance of Zn-O@Ag NPs was evaluated by the method of bacteriostatic ring and continuous LB medium dilution. The results show that the antibacterial performance of Zn-O@Ag-NPs is excellent, and the growth of the Zn-O@Ag-NPs has a certain inhibition effect on the growth of the yeast. The antibacterial performance of the Zn-O@Ag-NPs is close to that of the Ag-NPs in which the PVP is dispersed, but the content of the silver is only 25% of the latter; The MIC value of the yeast is 1.5 times that of the PVP-Ag NPs, which is 3 times that of the C-chitosan-Ag NPs, but the content of the silver is only 25% of the latter, so that the comprehensive inhibition effect on the yeast is also equivalent to that of the PVP-dispersed Ag NPs. The antibacterial mechanism of Ag NPs was also studied by CLSM. The antibacterial action of the Zn O@Ag NPs is mainly related to the oxidative damage of bacteria or fungal cells caused by a large amount of reactive oxygen species (ROS) inside the bacterial or fungal cells. In this paper, the toxicity of Zn-O-NPs, titanium dioxide nanoparticles (TiO2 NPs) and PVP-dispersed Ag-NPs in Chapter 2 was studied in this paper. The obvious inhibitory concentration of Zn O NPs, Ti O 2 NPs and Ag NPs on the multi-headed chorionic bacteria was 1750. m u.g/ m L,15000. m u.g/ m L and 300. m u.g/ m L, respectively. The toxicity of Ag NPs to the multi-headed chorionic bacteria was significantly stronger than that of Zn O NPs and Ti O2 NPs, and the minimum of Ti O2 NPs. Under the action of higher concentration of Zn-O NPs, the phenomenon of escape was found in the multi-headed suede, and the NPs and Ag-NPs of Ti-2 were not. Under the action of Zn-O-NPs, Ti-O2 NPs and Ag-NPs, the content of MDA in the multi-headed chorionic bacteria is dependent on the concentration of the nano-particles, and then the concentration of the nano-particles is increased, and then the concentration of the nano-particles is reduced, so that the degree of oxidation damage inside the original mass of the multi-head chorionic bacteria is more and more large, When increasing to a certain extent, the occurrence of the death of the multi-headed suede, and the decrease of the production of the MDA, the occurrence of the death of the multi-headed choriococcus has further provided the evidence for the ROS toxicity mechanism of the nano-particles.
【学位授予单位】：深圳大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TB383.1
【参考文献】
中国期刊全文数据库 前7条
1 史立平;李玉;;多头绒泡菌的生活史[J];东北师大学报(自然科学版);2010年04期
2 夏和生,王琪;纳米技术进展[J];高分子材料科学与工程;2001年04期
3 邓文雅;赵宗彬;沈琳;邱介山;;氧化锌空心球的制备及光致发光特征[J];功能材料;2007年09期
4 杨海朋;陈仕国;李春辉;陈东成;戈早川;;纳米电化学生物传感器[J];化学进展;2009年01期
5 沈兴海，高宏成;纳米微粒的微乳液制备法[J];化学通报;1995年11期
6 郭永，巩雄，杨宏秀;纳米微粒的制备方法及其进展[J];化学通报;1996年03期
7 袁金华;李光;陈慧珍;查河霞;宋黎军;;纳米氧化锌对人胚肺成纤维细胞的生物毒性[J];中山大学学报(医学科学版);2009年02期
中国硕士学位论文全文数据库 前3条
1 沈健;纳米技术进展研究[D];中南大学;2004年
2 张国山;纳米科技发展研究[D];武汉科技大学;2009年
3 高敏杰;银及氧化锌纳米材料的制备及抗菌性能[D];河南大学;2012年
，本文编号：2477636
本文链接：https://www.wllwen.com/kejilunwen/cailiaohuaxuelunwen/2477636.html
