几种新型固态抗菌材料的制备

发布时间：2019-05-23 20:22

【摘要】：微生物存在于生活境中的各个角落,以这样或那样的方式影响着我们的生活,他们之中有的是维持生态环境不可或缺的重要一环,有的则对人们的生命健康造成了严重的威胁,为了生存,人们不得不用尽各种方法去抵抗微生物对健康的侵蚀,抗生素是人们抵御病原微生物的重要武器,但细菌的耐药性已然成为一个日趋严峻的问题,新药开发的速度远远赶不上细菌产生耐药性的速度,要解决这一问题除了合理规范用药,坚持健康的生活习惯,就要从根本上减少人与病原微生物接触的机会-廉价高效的消毒剂的大范围使用是必然的,鉴于大部分消毒剂的强刺激性,在一些不适宜使用消毒剂的场合,在诸如公共健身休闲娱乐器材的表面使用温和、持久高效的高分子抗菌涂层就显得十分必要。本文尝试在两种不同的基质上枝接高分子季铵盐和多粘菌素,一种基质是以废弃的啤酒酵母为原料,通过水热反应得到的常温下十分稳定的两亲性酵母碳渣球,另一基质为通过水热反应合成的纳米四氧化三铁微球,在此基础上均以蒸馏沉淀法进一步枝接聚4-乙烯基吡啶,在混合溶剂中使用两种不同的卤化物与聚4-乙烯基吡啶成盐;以EDC、NHS作为活化剂将多粘菌素修饰在碳渣微球的表面,使用马尔文激光粒度仪,傅立叶红外光谱仪,扫描与透射电子显微镜,热重分析仪等分析检测仪器对修饰前后的碳渣微球、四氧化三铁微球的化学组成和微观形态进行检测分析。利用高效液相对碳渣微球表面的多粘菌素进行含量的测定,使用紫外分光光度法测定聚4-乙烯基吡啶修饰的微球表面的电荷数。以大肠杆菌和金黄色葡萄球菌为实验菌种,以蒸馏水为分散介质模拟自然水环境,对材料的抗菌活性进行测定。
[Abstract]:Microbes exist in every corner of life and affect our lives in one way or another. Some of them are indispensable to maintaining the ecological environment, while others pose a serious threat to people's lives and health. In order to survive, people have to use all kinds of methods to resist the erosion of health by microorganisms. Antibiotics are an important weapon for people to resist pathogenic microorganisms, but the drug resistance of bacteria has become an increasingly serious problem. The speed of new drug development is far from keeping pace with the drug resistance of bacteria. In addition to reasonably standardizing the use of drugs and adhering to healthy living habits, it is necessary to solve this problem. It is necessary to fundamentally reduce the chance of human contact with pathogenic microorganisms-the wide use of cheap and efficient disinfectants is inevitable, in view of the strong irritation of most disinfectants, in some cases where disinfectants are not suitable for use, It is necessary to use mild, lasting and efficient polymer antibacterial coating on the surface of public fitness leisure and entertainment equipment. In this paper, polymer quaternary ammonium salt and polymyxin were seeded on two different medium. one kind of matrix was a very stable Amphiphilic yeast carbon slag ball obtained by hydrothermal reaction using waste beer yeast as raw material. The other matrix is nanometer iron tetroxide microspheres synthesized by hydrothermal reaction, on the basis of which 4-vinylpyridine is further grafting by distillation precipitation method. Two different halides were used in the mixed solvent to form salt with poly4-vinylpyridine. Polymyxin was modified on the surface of carbon slag microspheres with EDC,NHS as activating agent. Ma Erwen laser particle size meter, Fourier transform infrared spectrometer, scanning and transmission electron microscope were used. The chemical composition and micromorphology of carbon slag microspheres and iron tetroxide microspheres before and after modification were detected and analyzed by thermogravimetric analyzer and other instruments. The content of polymyxin on the surface of carbon slag microspheres was determined by high efficiency liquid, and the charge number on the surface of poly4-vinylpyridine modified microspheres was determined by ultraviolet spectrophotometry. Escherichia coli and Staphylococcus aureus were used as experimental strains and distilled water as dispersion medium to simulate the natural water environment, and the antibacterial activity of the materials was determined.
【学位授予单位】：河南大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TB34

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