金纳米结构的制备及其表面增强荧光特性的研究

发布时间：2019-06-16 17:18

【摘要】：表面增强荧光是指具有特殊形貌及构型的金属表面能够使位于其临近的荧光分子的荧光信号发射强度较之自由态荧光发射强度大大增强的现象,作为一种重要的检测技术,表面增强荧光光谱分析法在生物学和生命科学中发挥着重要的作用。表面增强荧光的核心在于通过控制金属颗粒与荧光基团之间的距离,为了获得合适的增强效果,制备相应的纳米结构就成了重中之重。目前已经有很多不同的纳米金属结构制备成功,然而这些无序的纳米结构具有可控性与重复性差,不耐腐蚀,易发生表面结构形变等缺点,对表面增强荧光基底的商业化应用造成困难。针对于以上问题,本文提出一种具有荧光增强效果的金纳米阵列基底的制备方法,该基底是以表面具有微纳米结构的铝作为模板,配合以热蒸发、真空退火及磁控溅射技术,同时进一步引入石墨烯、二氧化硅等包覆材料,共同构成的复合结构,该方法可以在生命科学领域得到广泛的应用。本论文主要的创新点如下：1、突破传统氧化铝的限制,以去除氧化铝的铝作为基底制备的模板,该模板具有表面平整度高,结构清晰,均匀性好等优点,可以工业大规模生产；2、该结构在制备过程中创新性的使用了热退火技术,利用表面微纳米结构引导热退火的进程,以此完成对表面形貌的控制；3、引入石墨烯与二氧化硅等作为结构表面包覆材料,研究了两种材料与金纳米阵列复合后对荧光分子的荧光增强与猝灭作用,发现了二氧化硅包覆层具有有效的调节荧光基团与金纳米颗粒之间的距离、耐腐蚀、不易发生形变等优点,使基底具有在生物检测领域应用的潜力。
[Abstract]:Surface enhanced fluorescence refers to the phenomenon that the fluorescence signal emission intensity of the nearby fluorescent molecules can be greatly enhanced by the metal surface with special morphology and configuration compared with the free state fluorescence emission intensity. As an important detection technique, surface enhanced fluorescence spectroscopy plays an important role in biology and life science. The core of surface enhanced fluorescence is to control the distance between metal particles and fluorescent groups. In order to obtain the appropriate enhancement effect, the preparation of corresponding nanostructures has become the most important. At present, many different nanostructures have been successfully prepared. However, these disordered nanostructures have some shortcomings, such as poor controllability and reproducibility, corrosion resistance, easy to occur surface structure deformation and so on, which makes it difficult for the commercial application of surface enhanced fluorescent substrate. In order to solve the above problems, a preparation method of gold nanoarray substrate with fluorescence enhancement effect is proposed in this paper. the composite structure is composed of aluminum with micro-nanostructure on the surface, thermal evaporation, vacuum annealing and magnetron sputter, and further introducing graphene, silica and other coated materials. This method can be widely used in the field of life science. The main innovations of this paper are as follows: 1. Breaking through the limitation of traditional alumina, aluminum removal of alumina is used as the template prepared by substrate. The template has the advantages of high surface smoothness, clear structure, good uniformity and so on, so it can be produced on a large scale in industry. 2. The thermal annealing technology is used innovatively in the preparation process of the structure, and the surface microstructure is used to guide the thermal annealing process, so as to complete the control of the surface morphology. 3. Graphene and silica were introduced as structural surface coating materials, and the fluorescence enhancement and quenching of fluorescent molecules after recombination with gold nanoarray were studied. It was found that silica coating has the advantages of effectively adjusting the distance between fluorescent group and gold nanoparticles, corrosion resistance, not easy deformation and so on, which makes the substrate have the potential to be used in the field of biological detection.
【学位授予单位】：东南大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TB383.1;O614.123

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