对碘苯酚在金纳米微粒催化的化学发光体系的增强作用研究及其应用

发布时间：2021-12-01 22:09

　　化学发光（Chemiluminescence,CL）是化学反应过程中的一种光辐射现象。溶液相中的化学发光反应大多是基于氧化还原反应。在化学反应过程中,产物从反应中获得能量,从基态跃迁到激发态,分子从激发态回到基态的过程中以光子的形式释放能量,即化学发光。化学发光分析法是一种分子发光光谱分析方法,主要是基于待测化合物的浓度与在一定条件下的CL强度之间的线性关系进行定量分析。常见的化学发光分析体系有过氧草酸酯化学发光（Peroxyoxalate Chemiluminescence,PO-CL）体系、光泽精（Lucigenin）化学发光体系、联吡啶钌电致化学发光体系、鲁米诺（Luminol）化学发光体系等。其中,鲁米诺化学发光体系是最常用的一种化学发光体系,已被广泛应用于药物分析、环境分析和生物分析等领域测定不同的目标分析物,包括金属离子、葡萄糖、维生素、蛋白质、核酸、环境污染物等。然而,在碱性条件下,鲁米诺的自身化学发光反应强度非常弱。因此,发展有效的催化剂和增强剂对新型发光体系的发展及应用具有重要意义。鲁米诺体系常见的催化剂包括金属离子、天然酶、纳米材料等。而增强剂则主要用于过氧化物酶（... 

【文章来源】：天津大学天津市 211工程院校 985工程院校 教育部直属院校

【文章页数】：98 页

【学位级别】：硕士

【文章目录】：
中文摘要
ABSTRACT
Chapter1 Literature review
    1.1.Chemiluminescence and chemiluminescence analytical methods
    1.2.Common chemiluminescence analytical system
        1.2.1.Peroxyoxalate chemiluminescence system
        1.2.2.Acridinium salts chemiluminescence system
        1.2.3.Tris(2,2'-bipyridyl)ruthenium(II)chemiluminescence system
        1.2.4.Luminol chemiluminescence system
    1.3.Catalysts for luminol-H_2O_2 chemiluminescence system
        1.3.1.Metal ions(M~(n+))
        1.3.2.Peroxidase
        1.3.3.Nanomaterials(as mimetic peroxidase)
    1.4.Enhancers for luminol chemiluminescence system
        1.4.1.Fluorescein,naphthols,and hexahydroxybenzothiazole
        1.4.2.Substituted phenol derivatives
        1.4.3.Lophine derivatives and analogues
        1.4.4.Substituted boronic acid derivatives
        1.4.5.Water-soluble macromolecules and surfactants
        1.4.6.Co-enhancers
    1.5.The origin of my research work
Chapter2 Enhancement effect of p-iodophenol on gold nanoparticle-catalyzed chemiluminescence
    2.1.Introduction
    2.2.Experimental
        2.2.1.Apparatus and reagents
        2.2.2.Synthesis and characterization of Au NPs
        2.2.3.Procedure of CL measurement
    2.3.Results and discussions
        2.3.1.Characterization of Au NPs
        2.3.2.The enhancement effect of PIP on the luminol-H_2O_2-Au NPs CL system
        2.3.3.The enhancement effect of nine enhancers on the luminol-O_2-Au NPs CL system
        2.3.4.Kinetic curve of PIP-enhanced luminol-O_2-Au NPs CL system
        2.3.5.Optimization of the reaction conditions for the PIP-enhanced luminol-O_2-Au NPs CL system
        2.3.6.Catalytic activity of Au NPs with different diameters
        2.3.7.Selectivity of the proposed CL system
        2.3.8.Possible mechanism of the PIP-enhanced CL system
        2.3.9.Analytical applications of the proposed CL system
    2.4.Conclusions
Chapter3 Application of p-iodophenol-enhanced chemiluminescence reaction to immunoassay
    3.1.Introduction
    3.2.Experimental
        3.2.1.Apparatus and reagents
        3.2.2.Synthesis and characterization of Au NPs
        3.2.3.Preparation of Au NPs-labeled goat-anti-rabbit IgG secondary antibody..
        3.2.4.Assay procedure for CL detection of rabbit IgG
    3.3.Results and discussions
        3.3.1.Characterization of Au NPs
        3.3.2.Scheme for the detection of rabbit IgG
        3.3.3.Optimization of the assay conditions
        3.3.4.Assay performance for detection of rabbit IgG
        3.3.5.Selectivity of the proposed CL immunoassay system
        3.3.6.Analysis of rabbit IgG in the serum sample
    3.4.Conclusions
Chapter4 Conclusions and outlook
References
Published papers and scientific research
Acknowledgements


【参考文献】：
期刊论文
[1]苯酚和苯胺类化合物对光泽精化学发光的增强和抑制作用[J]. 石明娟,Aung Myint,董永平,崔华.  光谱学与光谱分析. 2007(10)
[2]金纳米粒子表面自组装巯基十一烷醇单分子层体系的制备及其光散射特性研究[J]. 尹洪宗,刘辉,李园园,何锡文,陈朗星,李文友.  化学学报. 2005(08)



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