非平面微流体芯片的设计、制备及复乳液微液滴的生成研究

发布时间：2018-02-24 06:23

本文关键词： 微流体聚二甲基硅氧烷复乳液超声成像　出处：《华东理工大学》2017年硕士论文　论文类型：学位论文

【摘要】：微流体芯片是芯片实验室(Lab on a chip)技术的一个重要分支。经过数十年的发展,微流体芯片已经发展成一种具备集成化、高通量、低成本的综合研究平台。微流体芯片的应用领域,横跨物理、生物、医药分析、微机电系统(MEMS)等领域,具有广阔的应用前景。微流体芯片的设计与制备,一直以来是微流体技术跨领域应用的研究要点。由于微流体的尺度问题,导致诸如毛细效应、扩散效应等问题;同时由于MEMS加工技术的限制,对于微流道的设计和加工的研究也比较单一,目前对于微流体通道的设计和制作,都是基于流体的二维方向。研究微流体芯片的设计和制备工艺,提出创新、高效的微流体设计方案,对微流体技术在各个领域的应用都起到促进作用。本文对微流道的数种平面结构设计方案进行了对比研究,并在此基础上,采用三维非平面结构设计的方法优化芯片结构,得到一种制作效率高、综合成本低、生产过程稳定的PDMS非平面芯片,并成功用于复乳液液滴的制备。同时,本文研究了微流体芯片的制作方法,针对非平面芯片制备时键合困难的问题,提出改良方案。针对常规微流体装置制作复杂的问题,本文设计了两种不依赖于超洁净环境和专门设备的微流体装置。本文介绍了此两种装置的制备方法,并通过实验验证了装置的可行性。最后,本文使用非平面芯片进行了复乳液液滴和微气泡的制备实验。实验证明了非平面芯片的实用性以及对复乳液液滴形态控制的可靠性。使用非平面芯片制备的复乳液微气泡,其尺寸变异系数小于1.5%,具有良好的形态学特性。通过生物体内超声造影实验,初步验证了复乳液微气泡用于超声造影诊断技术的可行性。
[Abstract]:Microfluidic chip is an important branch of Lab on a chip.After decades of development, microfluidic chip has developed into a kind of integrated, high-throughput microfluidic chip. The application field of microfluidic chip, covering physics, biology, medical analysis, micro electromechanical system (MEMS) and so on, has a broad application prospect, the design and manufacture of microfluidic chip, Because of the scale problem of microfluid, it leads to such problems as capillary effect, diffusion effect and so on. At the same time, because of the limitation of MEMS processing technology, At present, the design and fabrication of microfluidic channels are based on the two-dimensional direction of fluid. The highly efficient microfluidic design scheme promotes the application of microfluidic technology in all fields. In this paper, several kinds of planar structure design schemes of microfluidic channel are compared and studied, and on the basis of this, Using the method of three-dimensional non-planar structure design to optimize the chip structure, a kind of PDMS non-planar chip with high production efficiency, low synthesis cost and stable production process was obtained, and it was successfully used in the preparation of composite emulsion droplets. In this paper, the fabrication method of microfluidic chip is studied. Aiming at the difficulty of bonding in the fabrication of non-planar chip, an improved scheme is put forward. In this paper, two kinds of microfluidic devices are designed, which are not dependent on ultra-clean environment and special equipment. The preparation methods of these two devices are introduced, and the feasibility of the devices is verified by experiments. In this paper, the preparation experiments of composite emulsion droplets and microbubbles using non-planar chips are carried out. The practicability of the non-planar chips and the reliability of controlling the droplet morphology of the composite emulsion droplets are proved by the experiments. Its dimension variation coefficient is less than 1.5, and it has good morphological characteristics. The feasibility of using composite emulsion microbubbles in ultrasound diagnosis is preliminarily verified by the experiment of biologic in vivo contrast-enhanced ultrasound.
【学位授予单位】：华东理工大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TN492

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