液滴数字PCR芯片结果自动化读出平台的研究

发布时间：2018-03-01 14:07

本文关键词： ddPCR 核酸分子定量自动扫描液滴识别　出处：《现代电子技术》2017年18期 　论文类型：期刊论文

【摘要】：液滴数字聚合酶链式反应(ddPCR)作为第三代PCR技术,可实现核酸分子绝对定量。然而,在数字PCR结果读出过程中,对1 000万量级的液滴进行统计费时费力而且容易出错。针对此情况设计了液滴数字PCR芯片的自动扫描识别平台,系统包括硬件移动平台和软件拍照、拼接和识别功能。软件控制平移台顺序移动并控制摄像头拍照,移动完成后把拍得的照片拼接成一张图片,之后对图片中的液滴进行识别。此平台通过二维平移台的精确定位扫描及图像分割、识别算法,对荧光和非荧光液滴识别率达到99.6%以上。平台已经应用到了实验室中,对数字PCR芯片上的微液滴可实现一键识别,极大的减小了识别时间和错误率。
[Abstract]:As the third generation PCR technique, droplet digital polymerase chain reaction (DDP) can realize absolute quantification of nucleic acid molecules. However, in the process of reading out the results of digital PCR, It is time-consuming and error-prone to count 10 million droplets. An automatic scanning and identification platform for droplet digital PCR chip is designed in this paper. The system includes hardware mobile platform and software photography. The software controls the translation table to move sequentially and control the camera to take pictures. After the movement is completed, the photos are spliced into a picture. The platform has been applied to the laboratory, and the recognition rate of the fluorescent and non-fluorescent droplets is more than 99.6% through the precise location, scanning and image segmentation of the two-dimensional translation platform, and the recognition rate of the fluorescent and non-fluorescent droplets is more than 99.6%, and the platform has been applied to the laboratory. The microdroplets on the digital PCR chip can be recognized by one key, which greatly reduces the recognition time and error rate.
【作者单位】：上海大学量子与分子结构国际中心;中国科学院上海微系统与信息技术研究所;
【基金】：国家自然科学基金项目(21275153;61302052;61571428) 上海市浦江人才项目(15PJ1409800)
【分类号】：Q503;TN492

【相似文献】