便携芯片电泳非接触电导检测研究

发布时间：2018-03-11 03:17

本文选题：电泳芯片　切入点：非接触电导检测　出处：《华中科技大学》2015年硕士论文　论文类型：学位论文

【摘要】：全氟辛烷磺酰基化合物(PFOS)和全氟辛酸铵(PFOA)衍生物得到的共聚物在当今工业中得到广泛应用。研究表明,PFOS和PFOA类物质是危害人类健康的环境污染物,是全球斯德哥尔摩公约重点关注对象,但是目前还未找到完全合适的替代品,因此研究PFOS和和PFOA等全氟化合物的检测技术具有重要意义。目前PFOA及PFOS检测技术如光学检测、质谱检测等对测试样品要求高,且检测装置庞大,不利于现场测量。本文针对现场检测含氟化合物,提出非接触电导检测的微芯片电泳系统。论文首先比较了现有全氟化合物检测技术,再重点评述微流控芯片及其非接触电导检测技术的研究进展的基础上,用于分析全氟化合物提出的的便携式芯片电泳非接触电导检测仪器。其次,论文研究了非接触电导检测器的电路设计,包括检测器电路构成、激励信号选取和信号检测电路设计。本文选择高速函数发生器MAX038为核心并采用微控制器STM32作为电压控制系统,为检测器提供正弦激励交流信号;设计了基于选择电流反馈法的初级I-V转换电路、信号放大和整流电路,通过软件仿真,验证了非接触电导检测器的检测电路设计的可行性,并制作了检测电路PCB板,用于非接触电导检测。最后,论文构建了微流控芯片电泳非接触电导检测仪器试验测试平台。通过两电极非接触检测器电极系统的制作和电路测试,完成了不同溶液浓度的非接触电导测试。研究表明,本文设计的非接触电导检测器可有效感应出浓度变化范围为10-2mol/L~10-4 mol/L的变化,初步实现了预期的芯片电泳非接触电导检测装置的基本要求。
[Abstract]:The copolymers derived from perfluorooctanesulfonyl compounds (PFOS) and perfluorooctanoic acid (PFOAA) derivatives have been widely used in industry today. Studies have shown that perfluorooctanesulfonyl compounds and PFOA compounds are environmental pollutants harmful to human health. Is the focus of the Stockholm Convention, but no suitable alternatives have been found, so it is important to study the detection technology of perfluorinated compounds such as PFOS and PFOA. Currently, PFOA and PFOS detection techniques such as optical detection, Mass spectrometry has a high requirement for testing samples, and the equipment is too large, which is not good for field measurement. In this paper, fluorine compounds are detected in the field. A microchip electrophoresis system for contactless conductance detection is proposed. Firstly, the existing perfluorocarbon detection techniques are compared, and then the research progress of microfluidic chips and their non-contact conductance detection techniques are reviewed. A portable chip electrophoresis non-contact conductance detector proposed by perfluorinated compounds is analyzed. Secondly, the circuit design of non-contact conductance detector is studied, including the circuit structure of the detector. In this paper, the high speed function generator (MAX038) is chosen as the core and the microcontroller STM32 is used as the voltage control system to provide sinusoidal AC signal for the detector. The primary I-V conversion circuit, signal amplifying and rectifying circuit based on selective current feedback method are designed. The feasibility of the design of non-contact conductance detector detection circuit is verified by software simulation, and the PCB board of the detection circuit is made. Finally, a microfluidic chip electrophoretic non-contact conductivity testing instrument test platform was constructed. The fabrication and circuit test of the two-electrode non-contact detector electrode system were carried out. The contactless conductance measurements of different solution concentrations have been completed. The results show that the contactless conductance detector designed in this paper can effectively detect the change of concentration in the range of 10-2mol / L 10-4 mol/L. The basic requirements of the expected non-contact conductivity detector for chip electrophoresis have been preliminarily realized.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TN492;R927

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