CD-Like微流控生化分析系统光探测技术研究

发布时间：2018-05-06 16:51

本文选题：光探测 + 微流控芯片　；参考：《中国科学院研究生院(长春光学精密机械与物理研究所)》2015年硕士论文

【摘要】：CD-like微流控芯片是一种简单高效的生化分析平台,它通过调整自身的转速实现对芯片内部微流体的操作和控制,具有试剂用量少、集成度高、分析速度快、体积小等优点。本论文为实现CD-Like微流控芯片多指标、多波长的探测目的,围绕芯片的光探测系统设计,展开了以下研究工作:1.采用闪烁氙灯作为光源,微型光谱仪作为检测器,设计并搭建了光探测系统。验证了多路光电检测电路,给出了芯片精确定位与多参数检测方法;2.对影响光探测系统性能的主要因素进行了分析研究,给出了误差源,建立了光源输出稳定性、杂散光与吸光度误差之间的关系;给出了以双波长检测为主体的光源输出抖动补偿方法并对其进行了实验验证,相比单波长检测方法,检测结果的重复性提高了18倍;3.以葡萄糖、总蛋白、白蛋白和二氧化碳结合力等生化指标的检测为目标,对光探测系统性能进行了研究。测试结果显示,定标曲线的线性度达到99%以上,吸光度线性范围、重复性和杂散光均满足国家标准。本论文的工作将有利于推进CD-Like微流控芯片在医学临床领域的应用,对实现生化分析仪器的集成化与小型化具有重要意义。
[Abstract]:CD-like microfluidic chip is a simple and efficient biochemical analysis platform. It can operate and control the microfluid inside the chip by adjusting its rotational speed. It has the advantages of less reagent consumption, high integration, fast analysis speed and small volume. In order to realize the multi-target and multi-wavelength detection of CD-Like microfluidic chip, the following research work has been carried out in this paper, focusing on the design of the optical detection system of the chip. The light detection system is designed and built by using flash xenon lamp as light source and micro spectrometer as detector. The multichannel photoelectric detection circuit is verified, and the accurate location of the chip and the method of multi-parameter detection are given. The main factors affecting the performance of the optical detection system are analyzed and studied. The error source is given, and the relationship between the output stability of the light source, the stray light and the absorbance error is established. A method for compensating the output jitter of the light source with dual wavelength detection as the main part is presented and verified experimentally. The repeatability of the detection results is increased by 18 times and 3 times than that of the single wavelength detection method. The performance of the optical detection system was studied based on the detection of biochemical indexes such as glucose, total protein, albumin and carbon dioxide. The results show that the linearity of calibration curve is over 99%, and the linear range of absorbance, repeatability and stray light are all up to the national standard. The work of this paper will be helpful to promote the application of CD-Like microfluidic chip in the field of medical clinic, and it is of great significance to realize the integration and miniaturization of biochemical analysis instruments.
【学位授予单位】：中国科学院研究生院(长春光学精密机械与物理研究所)
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TN492

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