基于微环形阵列电极的细胞分离富集芯片的设计与实验研究

发布时间：2018-09-19 06:34

【摘要】：根据介电泳操作原理,设计了微环形阵列电极结构,建立了细胞分离富集芯片模型,采用COMSOL软件分析微环形阵列电极的电场分布和介电泳力方向并确定了最大和最小电场强度的位置,利用ITO玻璃和PDMS制备了细胞分离富集芯片。通过酵母菌细胞的介电泳富集实验和酵母菌细胞与聚苯乙烯小球的分离富集实验,明确了酵母菌细胞的临界频率,实现了酵母菌细胞和聚苯乙烯小球的分离富集。结果显示,在溶液电导率为60μs/cm,交流信号电压为8 Vp-p时,酵母菌细胞在1 k Hz~45 k Hz频率范围内做负介电泳运动并富集在环形内部,45 k Hz为酵母菌细胞的临界频率,在45 k Hz~10 MHz频率范围内做正介电泳运动并富集在环形边缘;1.5 MHz时聚苯乙烯小球做负介电泳运动并富集在环形内部,富集倍数达到11.66。
[Abstract]:According to the operation principle of dielectric electrophoresis, the structure of microannular array electrode was designed, and the model of cell separation and enrichment chip was established. The electric field distribution and the direction of dielectric electrophoresis force of microannular array electrode were analyzed by COMSOL software, and the position of maximum and minimum electric field intensity was determined. The cell separation and enrichment chip was prepared by ITO glass and PDMS. The critical frequency of yeast cells was determined and the separation and enrichment of yeast cells and polystyrene pellets were realized by the medium electrophoresis enrichment test of yeast cells and the separation and enrichment experiments of yeast cells and polystyrene pellets. The results showed that when the conductivity of the solution was 60 渭 s / cm and the AC signal voltage was 8 Vp-p, the yeast cells moved in negative medium electrophoresis in the frequency range of 1 k Hz~45 k Hz and enriched in the inner ring of 45 k Hz as the critical frequency of the yeast cells. In the frequency range of 45k Hz~10 MHz, positive electrophoretic movement was carried out and the polystyrene pellet was concentrated in the annular interior at 1.5 MHz at the circular edge, and the enrichment ratio was 11.666.The results showed that the electrophoretic activity of polystyrene pellets was negative in the range of 45 k Hz~10 MHz.
【作者单位】：中北大学电子测试技术重点实验室;仪器科学与动态测试教育部重点实验室;
【基金】：山西省百人计划西省基础研究项目(2014011021-3)
【分类号】：TN402

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