基于半导体胶体量子点荧光的多孔硅光学生物传感器的应用
发布时间：2019-04-07 18:38
【摘要】：多孔硅(PSi)是通过单晶硅上腐蚀出来具有海绵状结构的一维纳米光子晶体材料,PSi经过溶液处理后,其荧光背景低并且红外透光,易于制备成各种光子器件。利用不同机理的荧光与PSi光子器件结合,生物检测灵敏度被大大提升,可以使制备出的PSi光学生物传感器及生物芯片的性能更加优异。本论文主要将PSi优异的光学特性与半导体胶体量子点(QDs)的良好的生物特性相结合,设计并制备出高灵敏度的荧光光学生物传感器,并进行有效的生物检测,探索并制备出基于半导体胶体量子点荧光的多孔硅新型光学生物传感器。以下是本论文的主要研究内容和结论:1.通过偶联剂1-乙基-(3-二甲基氨基丙基)碳酰二亚胺盐酸盐(EDC)和N-羟基硫代琥珀酰亚胺(sulfo-NHS)激活水溶性CdSe/ZnS QDs的羧基,根据半导体胶体QDs的荧光峰设计出相应的PSi光子器件,半导体胶体QDs成功的与功能化后带氨基的PSi共价连接,增强了PSi内QDs的荧光强度。2.为了验证基于半导体胶体QDs荧光的多孔硅光学生物传感器的可行性,我们将半导体胶体QD标记的生物素、磷酸盐缓冲液(PBS)、及无标记的生物素添加到链霉亲和素修饰的PSi上,通过荧光对比,实验结果表明只有半导体胶体QD标记的生物素添加到链霉亲和素修饰的PSi内,才会产生荧光现象。从而证明基于量子点荧光的多孔硅光学生物传感器的可行性,进而对不同浓度的SA进行检测,其检出限为100pM。3.为了扩大利用半导体胶体量子点荧光的多孔硅光学生物传感器的应用,我们将该生物传感器检测自制的包虫病抗原。首先将半导体胶体QD标记包虫病抗体,然后与不同浓度的包虫病抗原修饰PSi进行生物反应。结果表明包虫病抗原浓度与PSi内QD免疫反应结合的荧光强度成线性关系,检出限为500fg/ml。
[Abstract]:Porous silicon (PSi) is a one-dimensional nano-photonic crystal material with spongy structure which is etched on monocrystalline silicon. After solution treatment, PSi has a low fluorescence background and infrared transmittance, so it is easy to be fabricated into various photonic devices. With the combination of different mechanisms of fluorescence and PSi photon devices, the sensitivity of biological detection is greatly improved, and the performance of the PSi optical biosensor and biochip can be made more excellent. In this thesis, the excellent optical properties of PSi and the good biological properties of semiconductor colloid quantum dot (QDs) are combined to design and prepare a high sensitivity fluorescent optical biosensor, and to carry out effective biological detection. A novel porous silicon biosensor based on semiconductor colloidal quantum dot fluorescence was developed. The following are the main contents and conclusions of this paper: 1. The carboxyl group of water-soluble CdSe/ZnS QDs was activated by coupling agent 1-ethyl-(3-dimethylaminopropyl) carbonyl diimine hydrochloride (EDC) and N-hydroxythiosuccinimide (sulfo-NHS). The corresponding PSi photon devices were designed according to the fluorescence peak of semiconductor colloid QDs. The semiconductor colloid QDs was successfully covalently connected with the functionalized PSi with amino group, which enhanced the fluorescence intensity of QDs in PSi. 2. In order to verify the feasibility of porous silicon optical biosensor based on semiconductor colloid QDs fluorescence, we labeled biotin and phosphate buffer (PBS), with semiconductor colloid QD. And non-labeled biotin was added to streptavidin-modified PSi. The results showed that only when the biotin labeled with semiconductor colloid QD was added to streptavidin-modified PSi, the fluorescence phenomenon could be produced. Thus, the feasibility of porous silicon optical biosensor based on quantum dot fluorescence is proved, and the detection limit of SA in different concentrations is 100pM.3. In order to expand the application of porous silicon optical biosensor using semiconductor colloid quantum dot fluorescence, we used the biosensor to detect the self-made antigen of hydatid disease. Firstly, semiconductor colloid QD was used to label the antibody against hydatid disease and then reacted with different concentrations of hydatid antigen modified PSi. The results showed that there was a linear relationship between the concentration of echinococcosis antigen and the fluorescence intensity of QD immunoreactivity in PSi, and the detection limit was 500 fg / ml.
【学位授予单位】：新疆大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O471.1;TP212.3
本文编号：2454317
[Abstract]:Porous silicon (PSi) is a one-dimensional nano-photonic crystal material with spongy structure which is etched on monocrystalline silicon. After solution treatment, PSi has a low fluorescence background and infrared transmittance, so it is easy to be fabricated into various photonic devices. With the combination of different mechanisms of fluorescence and PSi photon devices, the sensitivity of biological detection is greatly improved, and the performance of the PSi optical biosensor and biochip can be made more excellent. In this thesis, the excellent optical properties of PSi and the good biological properties of semiconductor colloid quantum dot (QDs) are combined to design and prepare a high sensitivity fluorescent optical biosensor, and to carry out effective biological detection. A novel porous silicon biosensor based on semiconductor colloidal quantum dot fluorescence was developed. The following are the main contents and conclusions of this paper: 1. The carboxyl group of water-soluble CdSe/ZnS QDs was activated by coupling agent 1-ethyl-(3-dimethylaminopropyl) carbonyl diimine hydrochloride (EDC) and N-hydroxythiosuccinimide (sulfo-NHS). The corresponding PSi photon devices were designed according to the fluorescence peak of semiconductor colloid QDs. The semiconductor colloid QDs was successfully covalently connected with the functionalized PSi with amino group, which enhanced the fluorescence intensity of QDs in PSi. 2. In order to verify the feasibility of porous silicon optical biosensor based on semiconductor colloid QDs fluorescence, we labeled biotin and phosphate buffer (PBS), with semiconductor colloid QD. And non-labeled biotin was added to streptavidin-modified PSi. The results showed that only when the biotin labeled with semiconductor colloid QD was added to streptavidin-modified PSi, the fluorescence phenomenon could be produced. Thus, the feasibility of porous silicon optical biosensor based on quantum dot fluorescence is proved, and the detection limit of SA in different concentrations is 100pM.3. In order to expand the application of porous silicon optical biosensor using semiconductor colloid quantum dot fluorescence, we used the biosensor to detect the self-made antigen of hydatid disease. Firstly, semiconductor colloid QD was used to label the antibody against hydatid disease and then reacted with different concentrations of hydatid antigen modified PSi. The results showed that there was a linear relationship between the concentration of echinococcosis antigen and the fluorescence intensity of QD immunoreactivity in PSi, and the detection limit was 500 fg / ml.
【学位授予单位】：新疆大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O471.1;TP212.3
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