基于光诱导介电泳力的微粒操纵研究
发布时间：2019-06-05 13:04
【摘要】：光诱导介电泳是基于介电泳基础上发展出来的一种新型微粒操纵技术，相对于传统的介电泳操纵，光电子镊最大的特点是可灵活操控的虚拟电极，通过软件控制可以在光电导层的任意位置形成需要的光学图案从而达成灵活的操纵效果。光诱导介电泳对微粒的无接触、无损伤的操纵，在生物科学、医学、药理学领域具有广泛的应用前景。 本文首先详细介绍了不同类型微纳操纵手段的理论与优缺点，随后介绍了光诱导介电泳的研究进展和在微纳操纵方面的应用，还对光诱导介电泳的理论进行了分析，在理论上推导了介电泳的操纵机制，，并使用COMSOL和MATLAB软件对其进行了分析，同时详述了光电导层的材料—非晶硅薄膜的结构、性能和常用制备方法。为后期淀积实验选择的合理参数提供了理论基础。最后对细胞的介电泳操纵进行研究，介绍了细胞的介电模型，和常用的介电分析方法。 在实验部分，首先是对光电导层的淀积实验，根据实际情况设定合适的参数，通过对淀积结果的测试，得出最优的一组参数。最后进行了试验台的搭建，设计了光诱导介电泳芯片，通过激发光电导层的光电效应形成虚拟电极，同时施加高频交流电压，实现了对4μm和10μm聚苯乙烯微球的介电泳力操纵。最终微球在负介电泳力的作用下分别以8.8μm/s和35μm/s的速度运动。通过光诱导介电泳实现了对微球的无接触无损伤的操纵。
[Abstract]:Photoinduced electrophoresis is a new particle manipulation technology based on dielectric electrophoresis. Compared with the traditional dielectric electrophoresis manipulation, optoelectronic tweezers are characterized by flexible virtual electrodes. The desired optical pattern can be formed at any position of the photoconductive layer by software control, so as to achieve flexible manipulation effect. Photoinduced electrophoresis has a wide range of application prospects in bioscience, medicine and pharmacology because of its non-contact and non-invasive manipulation of particles. In this paper, the theories, advantages and disadvantages of different types of micro-nano manipulation methods are introduced in detail, and then the research progress and application of photoinduced dielectric electrophoresis in micro-nano manipulation are introduced in detail, and the theory of photoinduced dielectric electrophoresis is also analyzed. The manipulation mechanism of dielectric electrophoresis is deduced theoretically and analyzed by COMSOL and MATLAB software. At the same time, the structure, properties and common preparation methods of amorphous silicon thin films, which are the materials of photoconductive layer, are described in detail. It provides a theoretical basis for the selection of reasonable parameters in the later deposition experiment. Finally, the manipulation of cell dielectric electrophoresis was studied, and the dielectric model of cells and the commonly used dielectric analysis methods were introduced. In the experimental part, first of all, the deposition experiment of the photoconductive layer is carried out, and the appropriate parameters are set according to the actual situation. Through the test of the deposition results, the optimal set of parameters is obtained. Finally, the test-bed is built, and the photoinduced dielectric electrophoresis chip is designed. The virtual electrode is formed by stimulating the photoelectric effect of the photoconductive layer, and the dielectric swimming force of 4 渭 m and 10 渭 m polystyrene microspheres is manipulated by applying high frequency AC voltage. Finally, the microspheres moved at the speeds of 8.8 渭 m / s and 35 渭 m / s, respectively, under the action of negative dielectric electrophoresis. The non-contact and damage-free manipulation of microspheres was realized by photoinduced electrophoresis.
【学位授予单位】：中北大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TN492
本文编号：2493550
[Abstract]:Photoinduced electrophoresis is a new particle manipulation technology based on dielectric electrophoresis. Compared with the traditional dielectric electrophoresis manipulation, optoelectronic tweezers are characterized by flexible virtual electrodes. The desired optical pattern can be formed at any position of the photoconductive layer by software control, so as to achieve flexible manipulation effect. Photoinduced electrophoresis has a wide range of application prospects in bioscience, medicine and pharmacology because of its non-contact and non-invasive manipulation of particles. In this paper, the theories, advantages and disadvantages of different types of micro-nano manipulation methods are introduced in detail, and then the research progress and application of photoinduced dielectric electrophoresis in micro-nano manipulation are introduced in detail, and the theory of photoinduced dielectric electrophoresis is also analyzed. The manipulation mechanism of dielectric electrophoresis is deduced theoretically and analyzed by COMSOL and MATLAB software. At the same time, the structure, properties and common preparation methods of amorphous silicon thin films, which are the materials of photoconductive layer, are described in detail. It provides a theoretical basis for the selection of reasonable parameters in the later deposition experiment. Finally, the manipulation of cell dielectric electrophoresis was studied, and the dielectric model of cells and the commonly used dielectric analysis methods were introduced. In the experimental part, first of all, the deposition experiment of the photoconductive layer is carried out, and the appropriate parameters are set according to the actual situation. Through the test of the deposition results, the optimal set of parameters is obtained. Finally, the test-bed is built, and the photoinduced dielectric electrophoresis chip is designed. The virtual electrode is formed by stimulating the photoelectric effect of the photoconductive layer, and the dielectric swimming force of 4 渭 m and 10 渭 m polystyrene microspheres is manipulated by applying high frequency AC voltage. Finally, the microspheres moved at the speeds of 8.8 渭 m / s and 35 渭 m / s, respectively, under the action of negative dielectric electrophoresis. The non-contact and damage-free manipulation of microspheres was realized by photoinduced electrophoresis.
【学位授予单位】：中北大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TN492
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