微流芯片中消逝波激励的荧光辐射特性研究
发布时间：2019-08-07 15:47
【摘要】：将石英裸光纤植入聚二甲基硅氧烷基片的微流道中,采用沿光纤轴向光抽运、消逝场激励染料分子的方式,在基片微流道中获得均匀的荧光辐射.实验发现,荧光辐射的强度随光纤轴向距离的增加而衰减,光纤包层溶液折射率越大,荧光沿光纤轴向的衰减越突出;包层溶液中染料浓度越大,荧光沿光纤轴向的衰减也越突出;通过选择适当的包层溶液折射率以及染料浓度可以获得沿光纤轴向接近均匀的荧光辐射.用消逝波激励荧光的辐射理论计算了荧光光强沿光纤轴向的变化,计算结果与实验符合较好.在此基础上,设计并制作了一种具有三个通道的聚二甲基硅氧烷基片,通过在三个微流道中分别注入染料浓度均为0.1 mmol的罗丹明640、罗丹明B及罗丹明6 G的乙醇染料溶液,采用沿光纤轴向消逝波光激励方式,在一块聚二甲基硅氧烷基片上同时实现了三个不同波段的荧光辐射.
[Abstract]:Quartz bare fiber was implanted into the microchannel of polydimethyl siloxane substrate. Uniform fluorescence radiation was obtained in the substrate microchannel by means of axial optical pumping along the optical fiber and excitation of dye molecules by vanishing field. It is found that the intensity of fluorescence radiation decays with the increase of axial distance of optical fiber, the larger the refractive index of optical fiber coating solution is, the more prominent the attenuation of fluorescence along the axial direction of optical fiber is, the higher the dye concentration in the coating solution is, the more prominent the attenuation of fluorescence along the axial direction of optical fiber is. By selecting the appropriate refractive index of coating solution and dye concentration, the fluorescence radiation close to uniform along the axial direction of optical fiber can be obtained. The variation of fluorescence intensity along the axial direction of optical fiber is calculated by using the radiation theory of vanishing wave excited fluorescence, and the calculated results are in good agreement with the experimental results. On this basis, a kind of polydimethyl siloxane substrate with three channels was designed and fabricated. Three different bands of fluorescence radiation were realized on a polydimethyl siloxane substrate by injecting Rhodamine 640, rhodamine B and rhodamine 6 G ethanol dye solutions with dye concentration of 0.1 mmol into three microchannels, and using the optical excitation mode of vanishing wave along the axial direction of the optical fiber, three different bands of fluorescence radiation were realized on a polydimethyl siloxane substrate at the same time. The fluorescence radiation in three different bands was realized at the same time by injecting Rhodamine 640, rhodamine B and rhodamine 6 G ethanol dye solutions into three microchannels.
【作者单位】： 云南大学物理系;
【基金】：国家自然科学基金(批准号:11404282,61465014) 中国科学院西部之光人才培养项目 云南大学中青年骨干教师培养项目资助的课题~~
【分类号】：TN492
本文编号：2524041
[Abstract]:Quartz bare fiber was implanted into the microchannel of polydimethyl siloxane substrate. Uniform fluorescence radiation was obtained in the substrate microchannel by means of axial optical pumping along the optical fiber and excitation of dye molecules by vanishing field. It is found that the intensity of fluorescence radiation decays with the increase of axial distance of optical fiber, the larger the refractive index of optical fiber coating solution is, the more prominent the attenuation of fluorescence along the axial direction of optical fiber is, the higher the dye concentration in the coating solution is, the more prominent the attenuation of fluorescence along the axial direction of optical fiber is. By selecting the appropriate refractive index of coating solution and dye concentration, the fluorescence radiation close to uniform along the axial direction of optical fiber can be obtained. The variation of fluorescence intensity along the axial direction of optical fiber is calculated by using the radiation theory of vanishing wave excited fluorescence, and the calculated results are in good agreement with the experimental results. On this basis, a kind of polydimethyl siloxane substrate with three channels was designed and fabricated. Three different bands of fluorescence radiation were realized on a polydimethyl siloxane substrate by injecting Rhodamine 640, rhodamine B and rhodamine 6 G ethanol dye solutions with dye concentration of 0.1 mmol into three microchannels, and using the optical excitation mode of vanishing wave along the axial direction of the optical fiber, three different bands of fluorescence radiation were realized on a polydimethyl siloxane substrate at the same time. The fluorescence radiation in three different bands was realized at the same time by injecting Rhodamine 640, rhodamine B and rhodamine 6 G ethanol dye solutions into three microchannels.
【作者单位】： 云南大学物理系;
【基金】：国家自然科学基金(批准号:11404282,61465014) 中国科学院西部之光人才培养项目 云南大学中青年骨干教师培养项目资助的课题~~
【分类号】：TN492
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