面向血红蛋白检测的石墨烯场效应晶体管纳米传感器研究

发布时间：2021-02-19 07:29

　　近年来,慢性病的患病率急剧上升,威胁着人类健康,影响着人们的生活质量。现有的临床检测技术成本高、耗时长,无法实现对慢性病的低成本快速检测。石墨烯场效应晶体管（Graphene Field Effect Transistor,GFET）纳米生物传感器具有灵敏度高、响应快等优点,在疾病预防和诊断中具有广阔的应用前景。本文介绍了一种基于适配型GFET传感器的平板液栅型GFET纳米生物传感器的设计与制作。通过理论分析和实验研究了核酸适体蛋白亲和结合对石墨烯中载流子浓度和Dirac点位置的影响,从而揭示了GFET纳米生物传感器对蛋白质生物标志物检测的传感机理。利用Hill方程和GFET输运特性建立了GFET纳米生物传感器响应信号与蛋白质浓度之间的数学模型,为解释核酸适体蛋白亲和结合效应对石墨烯漏源电流的影响提供了理论和实验支持。以血红蛋白分子为主要研究对象,利用GFET纳米生物传感器在人工磷酸盐溶液（PBS）和生物（血清）样本中对血红蛋白生物标记物进行了检测实验,以证明该核酸适配体与蛋白质分子具有良好的结合亲和力。利用拉曼光谱、能谱仪（Energy Dispersive Spectrometer... 

【文章来源】：哈尔滨工业大学黑龙江省 211工程院校 985工程院校

【文章页数】：64 页

【学位级别】：硕士

【文章目录】：
摘要
Abstract
CHAPTER1 INTRODUCTION
    1.1 Introduction and Motivations
    1.2 Research Background and Significance
        1.2.1 Graphene,its properties and production
        1.2.2 Graphene field effect transistors,their structure and benefits
        1.2.3 Fabrication of GFETs
        1.2.4 Aptamer
        1.2.5 Significance
    1.3 Hemoglobin Characterization
        1.3.1 Hemoglobin structure and function
        1.3.2 Conditions with a low Hemoglobin
        1.3.3 Conditions with an elevated Hemoglobin
        1.3.4 Abnormal Hemoglobin
    1.4 Main research contents
CHAPTER2 THEORETICAL ANALYSIS OF THE SENSING PRINCIPLE OF GFET NANOBIOSENSOR
    2.1 Introduction
    2.2 Electric transport characteristics of GFET sensor
    2.3 Principle of the nucleic acid aptamer GFET sensor detection
    2.4 Mathematical model of the GFET sensor response signal and Hb concentrations
    2.5 Summary
CHAPTER3 DESIGN AND FABRICATION OF THE GFET NANOBIOSENSOR
    3.1 Introduction
    3.2 The optimal transfer method of CVD graphene
    3.3 MEMS processing technology based design and fabrication of GFET sensor with a planar gate
    3.4 Characterization of the graphene functionalization
        3.4.1 Raman characterization of the PASE functionalization
        3.4.2 EDS characterization of the aptamer functionalization
        3.4.3 Electrical characterization of PASE and aptamer functionalization
    3.5 Summary
CHAPTER4 RESPONSE CHARACTERISTICS OF THE GFET NANOBIOSENSOR TO PROTEINS
    4.1 Introduction
    4.2 Protein detection in PBS using GFET nanobiosensor
        4.2.1 Control experiments of protein detection without and with aptamer functionalization
        4.2.2 Response characteristics of the GFET nanobiosensor to hemoglobin molecule
    4.3 Detection of Hemoglobin in Serum using GFET nanobiosensor
    4.4 Summary
CONCLUSION
REFERENCES
致谢



本文编号：3040795