近红外透射光谱的动物全血鉴别

发布时间：2017-12-26 19:08

本文关键词：近红外透射光谱的动物全血鉴别　出处：《光谱学与光谱分析》2016年01期 　论文类型：期刊论文

【摘要】：在进出口检测检疫部门,血液制品的检验与分类是件重要且复杂的事情。对于全血样品,开放式的采集可能带来污染,且血样中的致病因子可能会对检测人员造成危害。因此急需非接触式的全血分类鉴别方法。常用流式细胞术中的光谱方法由于需要对血细胞进行采样,所以无法在非接触全血分类鉴定中采用。红外吸收光谱学是一种可用来分析样品分子结构和化学键的技术,可以在不直接接触样品的情况下对样品进行探测。为寻找一种可实现非接触式血液样品种属差异性状探测的可行光谱方法,采用近红外谱段(4 497.669~7 506.4cm~(-1))对犬猫鸡三类常见动物全血样品进行了透射光谱测量。结果发现所测样品均在5 184~5 215cm~(-1)之间有个明显的吸收峰,在7 000cm~(-1)附近有个较平缓的吸收峰,且同种动物个体之间的透射光谱分布相似,只在整体透射率上有些差别。采用相关系数比较三类动物全血样品近红外透射光谱的区别,计算得出同种动物不同个体光谱曲线的相关系数均大于0.99,而不同种动物光谱曲线的相关系数在0.509 48~0.916 13之间。其中鸡与猫光谱曲线的相关系数在0.857 23~0.912 44之间;鸡与犬光谱曲线的相关系数在0.509 48~0.664 82之间;猫与犬光谱曲线的相关系数在0.872 75~0.916 13之间。犬猫同属哺乳纲,两者全血的近红外透射光谱相关系数比犬鸡或猫鸡非同纲动物的大,即光谱曲线的相似度更高。研究结果表明近红外透射光谱是一种非接触式动物全血鉴别的可行方法。
[Abstract]:In the import and export inspection and quarantine department, the inspection and classification of blood products is an important and complicated thing. For whole blood samples, open collection may bring pollution, and the pathogenic factors in blood samples may be harmful to the inspectors. Therefore, there is a need for non-contact classification of whole blood. The use of spectral methods in common flow cytometry can not be used in non-contact whole blood classification because it needs to sample blood cells. Infrared absorption spectroscopy is a technology that can be used to analyze the molecular structure and chemical bonds of samples. It can detect samples without direct contact with samples. To find a non-contact blood sample detection of different traits of varieties of feasible spectral methods using near infrared spectrum (4 497.669~7 506.4cm~ (-1)) of three kinds of common chicken dogs and cats animal blood samples to measure the transmission spectra. It was found that there was an obvious absorption peak between 5 184~5 215cm~ (-1) and a relatively gentle absorption peak near 7 000cm~ (-1). Correlation coefficients were used to compare the difference between near infrared transmission spectra of three kinds of animal blood samples. The correlation coefficients of different animal spectral curves were all greater than 0.99, while the correlation coefficients of different animal spectral curves were between 0.509 48~0.916 13. The correlation coefficient between chicken and cat spectral curve is between 0.857 23~0.912 44, and the correlation coefficient between chicken and dog spectral curve is 0.509 48~0.664 82, and the correlation coefficient between cat and dog spectral curve is 0.872 75~0.916 13. Dogs and cats are mammals, near infrared spectroscopy of both blood related coefficient than chicken or chicken dog cat with large animal classes, namely the higher similarity of spectral curve. The results show that the near infrared transmission spectrum is a feasible method for non-contact animal whole blood identification.
【作者单位】：中国科学院空间主动光电技术重点实验室;上海市动物疫病预防控制中心;
【基金】：国家自然科学基金项目(81260225) 国家高技术研究发展计划(863)项目(2015AA021103) 中国科学院“百人计划”择优项目资助
【分类号】：TS251.7;O657.33
【正文快照】： 引言全血样品的进出口检测检疫是件非常重要的事情。一方面,需要禁止国内特有动物的全血制品出口,因为其出口会造成国家物种资源信息的流失;另一方面,全血制品的进口必须加以检验及控制,以避免不明外来物种的入侵,造成生态环境的破坏。在进出口检验检疫部门,对于全血制品这种

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