基于微流控芯片的磁性没食子酸丙酯分子印迹聚合物的制备及应用

发布时间：2017-12-27 04:00

本文关键词：基于微流控芯片的磁性没食子酸丙酯分子印迹聚合物的制备及应用　出处：《分析化学》2016年04期 　论文类型：期刊论文

【摘要】：以3-(甲基丙烯酸酰氧)丙基三甲氧基硅烷修饰的Fe_3O_4为载体,没食子酸丙酯为模板分子,采用表面印迹技术制备了核壳结构的磁性没食子酸丙酯分子印迹聚合物(Fe_3O_4@SiO _2-MIPS),并将该磁性分子印迹聚合物作为吸附剂引入到微流控芯片中,与HPLC联用,实现对痕量酚类抗氧化剂的分离富集。采用扫描电镜、磁滞回线、热重分析、红外光谱及X射线衍射对该磁性分子印迹聚合物表征。在最佳萃取条件下(样品体积3 mL,样品溶液pH=5.0,样品流速为0.5 mL/min,洗脱液流速为0.3 mL/min),该聚合物对酚类抗氧化剂具有较强的富集作用。3种酚类抗氧化剂在10~5000 ng/mL范围内呈现良好的线性关系,线性相关系数均大于0.9901,日内和日间相对标准偏差(n=5)分别低于2.8%和3.5%。将本方法用于化妆水中酚类抗氧化剂的分析检测,加标回收率在87.9%~104.0%之间,相对标准偏差(n=3)小于3.7%。
[Abstract]:3- (methacrylic acyloxy) propyl trimethoxy silane modified Fe_3O_4 as carrier, propyl gallate as template molecule, magnetic gallate molecularly imprinted polymer core-shell structure were synthesized by surface imprinting technique (Fe_3O_4@SiO _2-MIPS), and the magnetic molecularly imprinted polymer as adsorption agent into a microfluidic chip, combined with HPLC, realize the separation of Trace Phenolic Antioxidants enrichment. The magnetic molecularly imprinted polymer was characterized by scanning electron microscopy (SEM), hysteresis loop, TG, IR and X ray diffraction. Under the optimum extraction conditions (sample volume 3 mL, sample solution pH=5.0, sample flow rate 0.5 mL/min, eluent flow rate 0.3 mL/min), the polymer has strong enrichment effect on phenolic antioxidants. 3 phenolic antioxidants showed a good linear relationship in the range of 10~5000 ng/mL, and the linear correlation coefficients were all greater than 0.9901, and the relative standard deviations (n=5) of intra day and inter day were less than 2.8% and 3.5%, respectively. This method is applied to the analysis and detection of phenolic antioxidants in make-up water. The rate of recovery is between 87.9%~104.0% and the relative standard deviation (n=3) is less than 3.7%.
【作者单位】：吉林大学化学学院;吉林大学生命科学学院;
【基金】：国家自然科学基金(No.21575049) 吉林省科技发展计划项目(No.20140101112JC)资助~~
【分类号】：O631.3
【正文快照】： 1引言微流控芯片是20世纪90年代迅速发展起来的一种分析技术,它主要通过微米级流路对流体进行操控达到微分析的目的[1,2]。微流控芯片装置可以实现集成化和自动化、减少试剂浪费、缩短分析时间、提高检测效率,引起了普遍的关注和兴趣。将微流控芯片用于样品前处理过程,不仅能

【相似文献】