基于四氧化三铁磁性纳米粒子的微囊藻毒素免疫传感器研究

发布时间：2018-01-02 00:05

本文关键词：基于四氧化三铁磁性纳米粒子的微囊藻毒素免疫传感器研究　出处：《分析化学》2016年05期 　论文类型：期刊论文

【摘要】：采用化学共沉淀法制备四氧化三铁(Fe_3O_4)磁性纳米粒子(MNPs),依次用3-氨基丙基三乙氧基硅烷(APTS)、丁二酸酐(SAH)对Fe_3O_4MNPs表面进行修饰,得到羧基功能化的核壳型磁性纳米粒子(Fe_3O_4@APTS·SAH MNPs),分别采用透射电镜(TEM)、磁滞回线、X射线光电子能谱(XPS)和傅里叶红外光谱(FTIR)对其进行了表征。将此纳米粒子修饰在自制的磁性玻碳电极(MGCE)表面,用1-(3-二氨基丙基)-3-乙基碳二亚胺盐酸盐(EDC)和N-羟基琥珀酰亚胺(NHS)活化纳米粒子表面的羧基,通过与氨基的共价交联,将抗微囊藻毒素-(亮氨酸-精氨酸)(MCLR)抗体(anti-MCLR)固定于该修饰电极上,用牛血清白蛋白(BSA)封闭非特异性吸附位点,构建了一种检测MCLR的电流型免疫传感器。采用直接竞争免疫反应模式,在标记物辣根过氧化物酶(HRP)的MCLR(MCLR-HRP)存在下,利用差分脉冲伏安法(DPV)测定溶液中的微囊藻毒素。在优化的实验条件下,免疫传感器对MCLR的线性测定范围为0.05~100μg/L,检出限为0.01μg/L(S/N=3)。构建的免疫传感器呈现出良好的重现性、稳定性和特异性。将本传感器用于实际水样的测定,加标回收率为94.3%~99.5%。
[Abstract]:Prepared by chemical coprecipitation Fe3O4 magnetic nanoparticles (Fe_3O_4) (MNPs), followed by 3- aminopropyltriethoxysilane (APTS), two (SAH) d anhydride modification on the surface of Fe_3O_4MNPs, obtained core-shell magnetic nanoparticles carboxyl functionalized (Fe_3O_4@APTS SAH MNPs), respectively by transmission electron microscopy (TEM), hysteresis loop, X ray photoelectron spectroscopy (XPS) and Fu Liye (FTIR) infrared spectra were characterized. The magnetic nanoparticles modified glassy carbon electrode (MGCE) surface, made by 1- (3- - propyl) -3- ethyl two imine hydrochloride (EDC) carbon and N-hydroxysuccinimide (N- NHS) activated nanoparticles through covalent cross-linking with carboxyl, amino, anti microcystin (leucine arginine) (MCLR) antibody (anti-MCLR) fixed on the modified electrode, using bovine serum albumin (BSA) to block the non-specific adsorption sites, The construction of an amperometric immunosensor for the detection of MCLR. By direct competitive immune response pattern, the markers of horseradish peroxidase (HRP) MCLR (MCLR-HRP) by using differential pulse voltammetry (DPV) determination of microcystins in solution. Under the optimized experimental conditions, the linear of immune sensor MCLR determination of the range of 0.05~100 g/L, the detection limit was 0.01 g/L (S/N=3). The immunosensor showed good reproducibility, stability and specificity. The sensor for the determination of real samples, and the recovery rate was 94.3%~ 99.5%.

【作者单位】：北京工业大学区域大气复合污染防治北京市重点实验室环境与能源工程学院;
【基金】：教育部博士点基金(No.20131103110011) 中国博士后科学基金(No.71005011201501) 北京市自然科学基金重点项目(B类)(No.KZ201110005006)资助~~
【分类号】：O657.1;TP212
【正文快照】： 1引言微囊藻毒素(MCs)是一种环状七肽肝毒素,可破坏人体细胞内的蛋白磷酸化平衡,引起肝脏病变[1,2]。目前,已发现80多种MCs的同分异构体[3],其中微囊藻毒素-(亮氨酸-精氨酸)(MCLR)是已发现的毒性最强的微囊藻毒素之一[4]。世界卫生组织(WHO)制定的《饮用水水质准则》[5]中规定

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