基于罗丹明6G和罗丹明B的新型铜离子荧光分子探针

发布时间：2018-01-02 04:03

本文关键词：基于罗丹明6G和罗丹明B的新型铜离子荧光分子探针　出处：《天津理工大学》2016年硕士论文　论文类型：学位论文

【摘要】：近些年,随着超分子化学的快速发展,分子识别已经成为生物、化学等领域的研究热点。根据识别主体和被识别客体之间相互作用时所产生的各种信号,如光、磁、电信号等,可以实现对客体的定性、定量检测,同时也可以作为研究其作用机理的有力工具。其中,基于光信号的检测方法,如紫外、荧光光谱等,由于其简单快捷、灵敏度高等方面的优势,在金属离子、生物分子等的检测方面有着重要的应用。铜作为生物体中的微量元素,在生物体的新陈代谢中有着至关重要的作用。但同时,铜离子作为一种环境污染物,对水体、土壤有着不利的一面。因此,能够简单、快速、灵敏的识别铜离子有着潜在的价值和重要的意义。在本论文中,分别以罗丹明6G和罗丹明B作为荧光信号基团,分别通过2-羟乙基肼和2-吡啶肼进行结构修饰,合成了四种新型铜离子荧光探针probe 1、2、3和4,实现了对铜离子的荧光“开启”型检测,并通过质谱说明识别过程的实质为铜离子促进的水解反应。在乙腈和水的混合溶剂中,四种探针都对铜离子表现出较高的选择性,并且有着明显的颜色变化。通过荧光光谱滴定得到了四种化合物对铜离子的荧光“开启”常数。四种探针都对铜离子有着较低的检测限,均在10~(-8) M数量级,其中probe 2的检测限达到10~(-10) M数量级,说明四种探针具有较高的灵敏度。同时,在竞争实验中,四种探针都表现出较好的抗干扰能力。在不同pH体系中的实验表明中性及弱酸、弱碱范围是探针分子识别铜离子的最佳pH条件。同时,probe 1和probe 3能够很好的在活细胞中对铜离子进行成像。这些良好的性质都为将probe 1至4应用于实际当中奠定了一定基础。
[Abstract]:In recent years, with the rapid development of supramolecular chemistry, molecular recognition has become a research hotspot in chemistry, biology, and other fields. According to the identification of the subject and identify all kinds of signals, the object of the interaction between the magnetic signals, such as light, etc., can achieve qualitative and quantitative detection of the object, but also can be used as a powerful tool study on the mechanism of action. The detection method based on optical signals, such as UV, fluorescence spectrum and so on, because of its simple and quick, high sensitivity advantage in metal ions, have important applications in detection of biological molecules. Copper as trace elements in organism, plays a crucial role in the organism in The new supersedes the old.. But at the same time, copper ion as an environmental pollutant on water and soil have a negative side. Therefore, to be simple, rapid and sensitive identification of copper ions has a potential The value and significance. In this paper, by Luo Danming 6G and Luo Danming B respectively as fluorescence signal groups, respectively by 2- and hydroxyethylhydrazine 2- pyridine hydrazine structure modification and synthesis of four new copper ion fluorescent probe probe and 1,2,3 4, the fluorescence of copper ion detection, and open "the essence of the recognition process for the hydrolysis reaction of copper ion by mass spectrometry. In promoting the mixed solvent of acetonitrile and water, four kinds of probes for copper ions exhibited higher selectivity, and has obvious color change. By fluorescence titration by fluorescence" four compounds of copper ion on "constant four. Probe of copper ion has a low detection limit were 10~ (-8) M order, of which 2 probe detection limit of 10~ (-10) M order, shows four kinds of probes with high sensitivity. At the same time, in the competition experiment In four, the probe has better anti-jamming capability. In different pH system experiments show that the neutral and weak acid, weak base is in the range of probe molecular recognition of copper ion optimum pH conditions. At the same time, probe 1 and probe 3 can be very good in living cells for copper ion imaging. These good properties for the probe 1 to 4 used to lay a certain foundation in practice.

【学位授予单位】：天津理工大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：O657.3

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