反相微乳法制备均一性水滑石及其在化学发光分析的应用

发布时间：2018-01-02 17:22

本文关键词：反相微乳法制备均一性水滑石及其在化学发光分析的应用　出处：《北京化工大学》2016年硕士论文　论文类型：学位论文

更多相关文章： 反相微乳 均一性 水滑石 化学发光

【摘要】：化学发光分析方法由于其灵敏度高、线性范围宽、仪器设备简单和易于实现自动化等优点而得到了广泛的研究和应用。然而,较低的发光强度在一定程度上限制其在分析化学领域中的应用。近些年,有关于纳米材料增敏化学发光的研究和探讨得到了广泛的关注。反相微乳法作为一种新型的无机纳米材料制备方法能够可控地合成粒径均一的纳米材料,并提高材料的应用性能。本论文通过反相微乳法可控地合成尺寸均一的水滑石纳米材料并用于增敏化学发光反应。主要的研究内容包括以下两个部分：1、以水滑石增敏鲁米诺-过氧化氢化学发光体系为例,采用反相微乳法制备了均一性不同的水滑石,首次探讨了纳米材料尺寸均一性对化学发光的影响。实验结果表明,水滑石的均一性对鲁米诺-过氧化氢化学发光反应体系的增敏起到了至关重要的作用。均一性好的水滑石由于具有更多的催化活性位点而更有利于增敏化学发光反应,并且在检测人体血浆样品中的葡萄糖含量时也得到了非常好的效果。此外,均一水滑石增敏发光反应的现象同样适用于其他发光体系(例如：过氧亚硝酸化学发光体系)。这些实验结果表明,纳米材料均一性依赖的化学发光具有通用性。2、采用反相微乳法合成尺寸均一的水滑石纳米材料并用于吸附磺基罗丹明101(S101)染料分子,所建立的S101@LDHs反相微乳体系能够显著地增敏草酸酯-过氧化氢化学发光体系。通过XRD、DLS、TEM、UV-vis、荧光偏振以及荧光寿命等手段表征和探究了增敏机理,并且该体系能够用于检测生物流体中的巯基氨基酸。
[Abstract]:Chemiluminescence analysis has been widely studied and applied due to its high sensitivity, wide linear range, simple instrument and easy automation. The low luminescence intensity limits its application in analytical chemistry to some extent. As a new preparation method of inorganic nano-materials, reverse microemulsion method can be used to synthesize nano-materials with uniform particle size in a controllable way. In this thesis, homogenous hydrotalcite nanomaterials were synthesized by inverse microemulsion method and were used to sensitize chemiluminescence reaction. The main research contents include the following two parts: 1. The hydrotalcite with different homogeneity was prepared by reverse microemulsion method with the example of hydrotalcite sensitized Luminol and hydrogen peroxide chemiluminescence system. The effect of size uniformity on chemiluminescence was investigated for the first time. The homogeneity of hydrotalcite plays an important role in sensitizing the luminol hydrogen peroxide chemiluminescence reaction system. The hydrotalcite with good homogeneity has more catalytic active sites, so it is more favorable to sensitize chemiluminescence. Reaction. And in the detection of human plasma samples of glucose content is also very good results. The phenomenon of homogeneous hydrotalcite sensitized luminescence reaction is also suitable for other luminescent systems (e.g. peroxynitrite chemiluminescence system). Homogenously dependent chemiluminescence (CL) of nanomaterials is universal. The homogenous hydrotalcite nanomaterials were synthesized by inverse microemulsion method and used to adsorb sulfonyl rhodamine 101 (S101) dye molecules. The reverse microemulsion system of S101 @ LDHs can significantly sensitize oxalate-hydrogen peroxide chemiluminescence system. Fluorescence polarization and fluorescence lifetime were used to characterize and explore the mechanism of sensitizing and the system could be used to detect mercapto amino acids in biological fluids.
【学位授予单位】：北京化工大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：O657.3

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