基于漫反射光谱的组织光学参数测量系统与方法研究

发布时间：2018-01-03 15:10

  本文关键词：基于漫反射光谱的组织光学参数测量系统与方法研究　出处：《光谱学与光谱分析》2016年05期 　论文类型：期刊论文

  更多相关文章： 组织光学参数 漫反射光谱 光纤探头 单一源探距离 主成分分析

【摘要】：在体组织光学参数测量是生物医学光子学研究重点,不仅为人体成分无创检测、光学成像、光动力疗法等研究提供基础,并且可以快速获取人体光学参数变化,为临床诊断提供依据。研究了利用单一源探距离漫反射光谱在体测量光学参数的测量系统与反构方法。漫反射光谱测量系统由宽谱光源、高分辨光纤光谱仪及光纤探头组成,结构简单,测量方便,可准确快速测量样品漫反射光谱。在光纤探头几何形状基础上,研究了光纤收集及系统传递函数,在此基础上对反构算法进行了校正。光学参数反构算法中正向模型基于Monte Carlo以及神经网络方法,适用光学参数范围大,计算速度快;逆向算法采用主成分分析与非线性建模拟合相结合的方法,可抑制测量噪声影响。在测量系统及反构算法基础上,进行了组织仿体光学参数测量实验,结果表明,利用单一源探距离下漫反射谱,可以较为准确获取吸收系数以及约化散射系数,均方根误差分别达到4.58%以及7.92%。为保证系统测量准确性,测量波长范围应覆盖样品中所含吸收物质吸收峰范围。所研究的在体组织光学参数测量方法为人体成分无创检测及测量条件变化获取提供了基础。
[Abstract]:The measurement of in vivo tissue optical parameters is the focus of biomedical photonics. It not only provides the basis for the study of human component noninvasive detection, optical imaging, photodynamic therapy, but also can quickly obtain the changes of human optical parameters. To provide the basis for clinical diagnosis. The measurement system and the inversion method of in vivo measurement of optical parameters using single source probe range diffuse reflectance spectroscopy are studied. The diffuse reflectance spectrum measurement system is based on a wide spectrum light source. The high resolution optical fiber spectrometer and the optical fiber probe are composed of simple structure and convenient measurement. Based on the geometrical shape of the optical fiber probe, the optical fiber collection and the system transfer function are studied. The forward model of optical parameter inversion algorithm is based on Monte Carlo and neural network method, which is suitable for a wide range of optical parameters and fast calculation speed. The inverse algorithm combines principal component analysis (PCA) with nonlinear modeling and fitting to suppress the influence of measurement noise. On the basis of the measurement system and the inverse algorithm, the measurement experiments of the optical parameters of tissue simulation are carried out. The results show that the absorption coefficient and the reduced scattering coefficient can be obtained more accurately by using the diffuse reflectance spectrum at a single source. The root mean square error is 4.58% and 7.92 respectively. The measurement wavelength range should cover the absorption peak range of the absorbent material contained in the sample. The optical parameter measurement method in vivo provides the basis for the noninvasive detection of human components and the acquisition of the change of measurement conditions.
【作者单位】： 天津大学精密测试技术及仪器国家重点实验室;天津大学精密仪器与光电子工程学院;
【基金】：国家(863)高技术研究发展计划项目(2012AA022602) 国家自然科学基金项目(81401454,81471698) 中国博士后科学基金项目(2013M541174) 中国博士后国际交流计划(20140066)资助
【分类号】：O657.33
【正文快照】： 引言随着光谱检测技术及仪器的发展,基于人体光谱测量的组织成分检测及成像诊断研究逐渐成为生物医学光学研究热点。人体组织光学特性,如吸收系数、散射系数变化与其生理病理状态密切相关,为肿瘤早期诊断、代谢动态监护及光动力治疗等临床应用提供了基础[1-5]。人体组织光学参，

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