气相色谱-质谱法测定食品中黄樟素及其衍生物含量

发布时间：2018-01-10 17:25

  本文关键词：气相色谱-质谱法测定食品中黄樟素及其衍生物含量　出处：《分析测试学报》2016年12期 　论文类型：期刊论文

  更多相关文章： 气相色谱-质谱(GC-MS) 选择离子扫描(SIM) 固相萃取(SPE) 黄樟素 香料添加剂 食品

【摘要】：建立了调味制品(调味料、番茄酱、酱油、花椒)、饮料(碳酸饮料、乳饮料、果汁)、肉制品(香肠、肉罐头、肉汤)、水果制品(果冻、果酱)、水产品(干鱼肉、鱼罐头、鱼肉泥)、小吃类(油炸薯片、糖果、巧克力)等18种食品中黄樟素、异黄樟素、二氢黄樟素含量的分析方法。样品在氯化钠盐析作用下,经二氯甲烷-乙腈提取,无水硫酸钠-氟罗里硅土柱吸附极性物质和水分。复杂样品需再经PRi ME HLB固相萃取柱(SPE)净化,5%苯基-95%甲基聚硅氧烷毛细管气相色谱柱分离,选择离子质谱技术监测,内标法定量。在最佳实验条件下,3种黄樟素在25~5 000μg/kg范围内呈良好的线性关系,相关系数(r2)≥0.9981,方法检出限(LOD)和定量下限(LOQ)分别为20μg/kg和50μg/kg,3个加标水平的回收率为77.6%~100.9%,相对标准偏差(RSD)为3.7%~13.6%。多种实际样品的测定结果表明,该方法准确可靠,适用于食品中3种黄樟素及其衍生物的定量和定性分析。
[Abstract]:Seasoning products (seasoning, ketchup, soy sauce, prickly ash, soft drinks (carbonated drinks, milk drinks, juices), meat products (sausage, canned meat, broth, fruit products (jelly, jam)) were established. Aquatic products (dried fish, canned fish, fish paste, snacks (fried potato chips, candy, chocolate) and other 18 kinds of food safrole, isosafrole. Method of analysis of dihydrosafrole content. The sample was extracted by dichloromethane-acetonitrile under the action of sodium chloride salting out. Anhydrous sodium sulfate-fluorosilicate column was used to adsorb polar substances and water. The complex samples need to be purified by PRi ME HLB solid phase extraction column (SPE). 5% phenyl 95% methyl polysiloxane was separated by capillary gas chromatography and monitored by ion-mass spectrometry. There was a good linear relationship among the three safrole compounds in the range of 25 渭 g / kg, the correlation coefficient (r _ 2) 鈮，

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