基于全血可见吸收光谱的物种鉴别

发布时间：2018-01-31 21:40

本文关键词： 吸收光谱可见光主成分分析正态分布　出处：《光谱学与光谱分析》2017年11期 　论文类型：期刊论文

【摘要】：血液及血液制品是一种特殊的商品,在流通过程中需要严加管控。传统的方法通常为应用特征抗体检测人血红蛋白,或应用液相色谱(HPLC)、质谱(MS)等方法对血液物种进行鉴别,鉴别过程中外界环境和血液样本会发生接触。为了防止血液样本和外界环境相互污染,因此需要发展非接触式的血液物种鉴别技术。设计了一种基于吸收光谱的血液物种鉴别方法。实验测试了人与牛全血样本共24份,每种样本各10份,另有4份标记为未知样本。装有血液样本的PET管置于积分球内,使用超连续谱光源对积分球进行照射,测量420~740nm范围内匀光后的吸收光谱,每个样品测试5次取平均。实验测试了使用主成分分析法对测得的原始光谱数据进行降维。并对降维后的数据应用二维正态分布进行拟合。通过样本在置信空间内的概率分布或到已知分类中心的距离对未知样本的物种进行鉴别。可以得出结论,应用PCA和二维正态分布拟合的方法,能够较为精确地实现对全血的可见吸收光谱物种鉴别。
[Abstract]:Blood and blood products are special commodities which need to be strictly controlled during circulation. The traditional methods are to use characteristic antibodies to detect human hemoglobin or to use liquid chromatography (HPLC). In order to prevent the blood samples from polluting each other, the blood species can be identified by mass spectrometry (MS), and the external environment and blood samples will come into contact in the process of identification. Therefore, it is necessary to develop non-contact blood species identification technology. A blood species identification method based on absorption spectrum is designed. 24 human and bovine whole blood samples were tested, with 10 samples each. The other 4 samples were labeled as unknown samples. The PET tubes containing blood samples were placed in the integral sphere, and the absorption spectra of the integrated spheres were measured by using supercontinuum spectral light source to measure the absorption spectra after uniform light in the range of 420 ~ 740 nm. The principal component analysis (PCA) was used to reduce the dimension of the measured original spectral data. The reduced dimension data were fitted with two-dimensional normal distribution. The samples were measured in confidence space. The probability distribution within or the distance from the known taxonomy centers to identify the species of unknown samples. Conclusion can be drawn. The method of PCA and two-dimensional normal distribution fitting can accurately identify the species of visible absorption spectrum of whole blood.
【作者单位】：中国科学院上海技术物理研究所空间主动光电技术重点实验室;
【基金】：国家高技术研究发展计划(863计划)项目(2015AA021103) 国家自然科学基金项目(81260225,51428302) 中国科学院重点实验室基金项目(CXJJ-14-S134);中国科学院“百人计划”择优项目资助
【分类号】：O657.3;Q5-3
【正文快照】： 引言血液及血液制品是一种特殊的商品。血液中携带着重要的物种资源遗传信息,如果不经管控自由出口,会直接导致我国生物遗传资源信息的流失,如果这些资源被别有用心的人士利用,可能开发出针对我国特有生物的药物或生物武器。另一方面,血液及血液制品的进口也有一定风险,如果不

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