化学修饰电极在食用色素中的研究与应用

发布时间：2018-02-06 03:17

本文关键词： 石墨烯精氨酸日落黄诱惑红柠檬黄胭脂红苋菜红修饰电极循环伏安法差分脉冲伏安法　出处：《淮北师范大学》2017年硕士论文　论文类型：学位论文

【摘要】：本文采用循环伏安法(CV)制备了聚L-精氨酸/石墨烯修饰玻碳电极(PLA-ERGO/GCE),并用循环伏安法(CV)和差分脉冲伏安法(DPV)探究了日落黄(SY)、诱惑红(AR)、柠檬黄(TT)、胭脂红(CA)和苋菜红(AT)在PLA-ERGO/GCE修饰电极上的电化学行为,分别建立了单独测定SY、AR、TT以及同时测定CA和AT的新方法。用扫描电镜(SEM)表征手段探究了修饰电极的表面形貌,采用交流阻抗技术(EIS)对电极进行表征。通过对电极表面的功能化设计,从而建立了用于食品中合成色素检测的新型化学修饰电极,将此修饰电极用于各样品中含量的测定,结果满意。在整个研究过程中,主要解决的问题有:(1)聚L-精氨酸/石墨烯修饰电极(PLA-ERGO/GCE)的制备条件及表征;(2)在PLA-ERGO/GCE修饰电极上分别探讨了日落黄、诱惑红、柠檬黄、胭脂红和苋菜红的电化学行为;(3)在PLA-ERGO/GCE修饰电极上分别探讨了日落黄、诱惑红、柠檬黄的最佳测定条件,建立了测定方法;(4)在PLA-ERGO/GCE修饰电极上探讨了同时测定胭脂红和苋菜红的新方法。主要研究内容如下:1、采用循环伏安法(CV)制备聚L-精氨酸/石墨烯修饰电极(PLA-ERGO/GCE),研究了日落黄(SY)在此修饰电极上的电化学行为,建立了测定SY的新方法。在p H3.5的磷酸盐缓冲溶液中,扫描速率为100m V/s时,日落黄在0.826V处产生一个灵敏的氧化峰,其峰电流与日落黄的浓度在7.50×10-7~1.00×10-3mol/L范围内呈现良好的线性关系,检出限为1.0×10-7 mol/L。用于实际样品中日落黄的分析与测定,结果令人满意。2、通过循环伏安法(CV)制备聚L-精氨酸/石墨烯修饰电极(PLA-ERGO/GCE),实现了对诱惑红(AR)的单独测定,在p H为3.5的磷酸盐缓冲溶液中,当扫速为100m V/s时,诱惑红产生一对氧化还原峰,其中氧化峰位于0.276V处,还原峰位于0.086V处。运用循环伏安法测定AR时,AR的氧化峰电流与其浓度在7.50×10-7~1.00×10-4mol/L范围内有良好的的线性关系,检出限为1.0×10-8mol/L。用于样品中诱惑红的分析与测定,结果令人满意。3、通过电化学方法制得了聚L-精氨酸/石墨烯修饰电极(PLA-ERGO/GCE),运用循环伏安法(CV)和差分脉冲伏安法(DPV)探究了柠檬黄在该化学修饰电极上的电化学行为,在p H为2.0的磷酸盐缓冲溶液中,当扫速为100m V/s时,柠檬黄在1.012V处出现一个灵敏的氧化峰。用DPV法测定时,柠檬黄的氧化峰电流与其浓度在1.00×10-6~2.50×10-4mol/L范围内呈现良好的线性关系,检出限为2.5×10-7mol·L-1。用于实际样品中柠檬黄的分析与测定,取得令人满意的结果。4、利用PLA-ERGO/GCE修饰玻碳电极探究了胭脂红(CA)和苋菜红(AT)共存时的电化学行为,建立了同时测定胭脂红和苋菜红的新方法。研究结果表明,在p H2.5的磷酸盐缓冲溶液中,120m V/s的扫速下,胭脂红产生一对氧化还原峰,其中氧化峰位于0.763V处,还原峰位于0.658V处;苋菜红在0.884V处产生一个氧化峰。胭脂红和苋菜红两者的氧化峰电位差达到121m V,而且不相互干扰,因此可以用于胭脂红和苋菜红的同时测定。在最佳的实验条件下,用差分脉冲伏安法(DPV)同时测定CA和AT的线性范围均为7.50×10-7~7.50×10-5mol/L,检出限分别为7.5×10-8mol/L和2.5×10-7mol/L。将其用于样品中胭脂红和苋菜红的测定,结果满意。
[Abstract]:In this paper, using cyclic voltammetry (CV) poly arginine L- / graphene modified glassy carbon electrode was prepared (PLA-ERGO/GCE), and (CV) by cyclic voltammetry and differential pulse voltammetry (DPV) on sunset yellow (SY), Allura red (AR), lemon yellow (TT). Carmine (CA) and amaranth (AT) on the electrochemical behavior of PLA-ERGO/GCE modified electrode, respectively, established a separate determination of SY, AR, TT and the new method for simultaneous determination of CA and AT. By using scanning electron microscopy (SEM) characterization of the surface morphology of the modified electrode, using AC impedance technique (EIS) for the characterization of the electrode. Through the functional design of the electrode surface, thus established a new chemically modified electrode synthetic pigments in food detection, the modified electrode for determination of content in each sample with satisfactory results. In the process of study, the main problems are: (1) L- / poly arginine graphene modified electrode (PLA-E Preparation and characterization of RGO/GCE); (2) in the PLA-ERGO/GCE modified electrode were discussed respectively. The temptation of red, sunset yellow, lemon yellow, carmine and amaranth electrochemical behavior; (3) red temptation in the PLA-ERGO/GCE modified electrode were studied, the best conditions for determination of sunset yellow, lemon yellow, set up determination method; (4) in the PLA-ERGO/GCE modified electrode was discussed and a new method for the determination of carmine and amaranth. The main contents are as follows: 1, using cyclic voltammetry (CV) preparation of poly L- arginine / graphene modified electrode (PLA-ERGO/GCE), sunset yellow (SY) on the electrochemical behavior of this the modified electrode, a new method was established for the determination of SY. In P phosphate buffer solution H3.5, scan rate of 100m V/s, sunset yellow at 0.826V produces a sensitive oxidation peak, the peak current and the concentration of sunset yellow in the range of 7.50 * 10-7~1.00 * 10-3mol/L There was a good linear relationship, detection limit of 1 * 10-7 mol/L. for analysis and determination in real samples with satisfactory results, and the.2 by cyclic voltammetry (CV) preparation of poly L- arginine / graphene modified electrode (PLA-ERGO/GCE), the temptation of red (AR) alone determined. In P H 3.5 phosphate buffer solution, when the scanning rate was 100m V/s, Allura red, a pair of redox peaks, the oxidation peak is located at 0.276V, the reduction peak is located at 0.086V. The determination of AR by cyclic voltammetry, the oxidation peak current of AR and its concentration has a good linear relationship in the range 7.50 * 10-7~1.00 * 10-4mol/L, a detection limit of 1 * 10-8mol/L. for analysis and determination of Allura red in samples with satisfactory results,.3, was prepared by electrochemical method of poly L- arginine / graphene modified electrode (PLA-ERGO/GCE), by using cyclic voltammetry (CV) and differential pulse Voltammetry (DPV) on the electrochemical behavior of lemon yellow in the chemically modified electrode, in P H 2 phosphate buffer solution, when the scanning rate was 100m V/s, lemon yellow 1.012V is a sensitive oxidation peak. Measured by DPV, the oxidation peak current of lemon yellow and the concentration showed a good linear relationship in the range of 1 * 10-6~2.50 * 10-4mol/L, a detection limit of 2.5 * 10-7mol L-1. for analysis and determination of tartrazine in actual samples, obtained satisfactory results of.4, explores the use of carmine PLA-ERGO/GCE modified glassy carbon electrode (CA) and amaranth (AT) electrochemical behavior of coexistence the new method has been developed for the simultaneous determination of carmine and amaranth. The results showed that in P phosphate buffer solution H2.5, scan speed 120m V/s, carmine a pair of redox peaks, the oxidation peak is located at 0.763V, the reduction peak is at 0.658 V; amaranth produces an oxidation peak at 0.884V. The oxidation peak potential of carmine and amaranth, the difference between the two reached 121m V, and does not interfere with each other, so it can be used for the determination of carmine and amaranth at the same time. Under the optimum conditions, using differential pulse voltammetry (DPV) linear range simultaneous determination of CA and AT were 7.50 * 10-7~7.50 * 10-5mol/L, the detection limits were 7.5 * 10-8mol/L and 2.5 * 10-7mol/L. for the determination of carmine and amaranth in samples with satisfactory results.

【学位授予单位】：淮北师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TS264.4;O657.1

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