pH敏感纳米凝胶的制备及其在siRNA转染中的应用

发布时间：2018-02-06 05:43

本文关键词： 葡聚糖纳米凝胶基因传递 pH敏感　出处：《高分子学报》2017年07期 　论文类型：期刊论文

【摘要】：通过分散聚合的方法,以改性了双键的葡聚糖(Dex-AA)作为交联剂,甲基丙烯酸二甲氨基乙酯(DMAEMA)作为单体,过硫酸铵(APS)和四甲基乙二胺(TEMED)分别作为引发剂和助引发剂,合成了不同交联度的、具有pH敏感内吞增强作用的葡聚糖纳米凝胶(DD-NGs),并测试了其复合siRNA进行转染的能力.实验结果表明,该纳米凝胶表面带有正电荷,具有较好的担载siRNA进入肿瘤细胞并沉默基因的能力,且具有pH响应粒径变化的性质.在pH=7.4的体液环境中,纳米凝胶与基因的复合物粒子较小;在肿瘤酸性(pH=6.8)条件下,纳米凝胶与基因的复合物粒子变大,显著地增强了肿瘤细胞对纳米凝胶与基因复合物的内吞.
[Abstract]:The modified dextran (Dex-AA) was used as crosslinking agent and dimethylaminoethyl methacrylate (DMAEMA) was used as monomer by dispersion polymerization. Ammonium persulfate (APS) and tetramethyl ethylenediamine (TEMED) were used as initiators and co-initiators, respectively. The pH sensitive endocytosis enhanced dextran nanogel DD-NGsN was tested and its ability to transfect the composite siRNA was tested. The experimental results showed that the surface of the nanogel was positively charged. It has the ability of carrying siRNA into tumor cells and silencing genes, and has the property of pH response to particle size change. In the humoral environment of pH=7.4. The composite particles of nanogel and gene were smaller. Under the condition of tumor acidic pH = 6.8), the complex particles of nano-gel and gene became larger, which significantly enhanced the endocytosis of tumor cells to nano-gel and gene complex.
【作者单位】：中国科学院长春应用化学研究所生态环境高分子材料重点实验室;中国科学院大学;长春市朝阳区人民医院;
【基金】：国家自然科学基金(基金号21474104,51233004,51520105004,51390484) 中共中央组织部“万人计划”青年拔尖人才项目吉林省重点科技攻关项目(项目号20160204032GX)资助
【分类号】：O648.17;R450

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