高效液相色谱-荧光衍生法快速测定地沟油中的长链脂肪醛

发布时间：2018-02-09 19:31

本文关键词： 地沟油长链脂肪醛荧光衍生化高效液相色谱法　出处：《分析化学》2017年05期 　论文类型：期刊论文

【摘要】：本研究利用地沟油精炼过程中会产生醛类化合物的特点,以O-(3-(9H-咔唑-9-基)丙基)羟胺为荧光衍生化试剂,采用高效液相色谱法测定己醛、庚醛、辛醛、壬醛、癸醛5种长链脂肪醛来鉴别地沟油及食用油中是否掺杂地沟油。10μL待测油样品溶于200μL异丙醇,在最佳条件下与O-(3-(9H-咔唑-9-基)丙基)羟胺反应生成稳定的衍生物,乙腈萃取后进样分析。以乙腈-水(90:10,V/V)为流动相,在λ_(ex)/λ_(em)=292 nm/348 nm下荧光检测,衍生物在C_(18)柱上15 min内可完全分离。检测5种脂肪醛的线性范围均为0.01~1.00μmol/L,检出限为0.05~0.10 nmol/L,加样回收率为95.6%~101.4%。待测油样品的测定结果表明,食用油基本不含上述5种脂肪醛,而精炼地沟油中5种脂肪醛含量显著增加,因此5种脂肪醛含量可作为检测地沟油的指标。本方法专属性强、灵敏度高、准确性好、方便、快捷,可用于快速检测地沟油。
[Abstract]:In this study, the characteristics of aldehydes produced in the refining process of gutter oil were used to determine hexanal, heptanal, octylaldehyde, nonaldehyde, hexanal, heptanal, octylaldehyde and nonaldehyde by high performance liquid chromatography (HPLC), using O-Pn-9H-carbazole-9- (propyl) hydroxylamine as fluorescent derivatization reagent. Five long-chain aliphatic aldehydes of decanal were used to identify whether the gutter oil or edible oil was doped in the sample of gutter oil. 10 渭 L oil was dissolved in 200 渭 L isopropanol. Under the optimum conditions, the sample reacted with O-C3- (3-Carbazole-9-) propyl) hydroxylamine to form a stable derivative. Sample analysis after acetonitrile extraction. Using acetonitrile-water 90: 10 V / V) as mobile phase, fluorescence detection was carried out at 位 _ (+) / 位 ~ (-1) / 位 ~ (-1) nm/348 _ (2) nm. Derivatives can be completely separated within 15 min on CST18) column. The linear range for the determination of five aliphatic aldehydes is 0.01 ~ 1.00 渭 mol 路L ~ (-1), the detection limit is 0.05 ~ 0.10 nmol / L, the recovery rate is 95.6% ~ 101.4%. The results of the determination of the oil samples show that the edible oils basically do not contain the five kinds of fatty aldehydes. The content of five aliphatic aldehydes in refined gutter oil increased significantly, so the five kinds of aliphatic aldehydes can be used as indicators for the detection of gutter oil. This method has the advantages of high specificity, high sensitivity, good accuracy, convenience and rapidity, and can be used for rapid detection of gutter oil.
【作者单位】：中国药科大学理学院;药物质量与安全预警教育部重点实验室;
【基金】：江苏省自然科学基金项目(No.BK20140654)资助
【分类号】：O657;TQ646

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