固相萃取-气相色谱法测定黄酒中β-苯乙醇含量

发布时间：2018-02-11 19:47

本文关键词： 固相萃取气相色谱黄酒 β-苯乙醇　出处：《食品科学》2017年24期 　论文类型：期刊论文

【摘要】：目的:建立黄酒中β-苯乙醇的固相萃取-气相色谱检测方法。方法:HLB型固相萃取小柱经活化、平衡后,对3.0 mL黄酒样品中的β-苯乙醇进行富集,依次以3.0 mL水、3.0 mL体积分数40%甲醇溶液淋洗小柱,用3.0 mL甲醇洗脱样品,用气相色谱-氢火焰离子化检测器对目标物进行检测,外标法定量。结果:β-苯乙醇在10.1~202.0 mg/L质量浓度范围内,线性相关系数(r)为0.999 9,检出限(RSN=3)和定量限(RSN=10)分别为0.9 mg/L和3.0 mg/L,回收率在95.5%~98.5%之间,相对标准偏差为1.1%~1.4%。结论:该方法具有操作便捷、结果准确的优点,同时有效降低了黄酒基质对气相色谱系统的污染,避免了酒样中水分对毛细管色谱柱的危害,适用于黄酒中β-苯乙醇的检测。
[Abstract]:Objective: to establish a solid phase extraction-gas chromatographic method for the determination of 尾 -phenylethanol in rice wine. Methods: after activation and equilibrium, 尾 -phenylethanol was enriched in 3.0 mL yellow rice wine sample. The sample was eluted by 3.0 mL methanol with 3.0 mL water and 3.0 mL volume fraction of 40% methanol. The target was detected by gas chromatography-hydrogen flame ionization detector. Results: the linear correlation coefficient of 尾 -phenylethanol was 0.999 9 in the concentration range of 10.1 ~ 202.0 mg/L, the detection limit was 0.999 9, and the quantitative limit was 0. 9 mg/L and 3. 0 mg / L, respectively. The recovery was between 95. 5% and 98. 5%. Conclusion: this method has the advantages of convenient operation and accurate results. At the same time, it can effectively reduce the pollution of rice wine matrix to the gas chromatographic system and avoid the harm of the moisture in the wine sample to the capillary column. It is suitable for the detection of 尾-phenylethanol in rice wine.
【作者单位】：西安市产品质量监督检验院;
【基金】：国家质量监督检验检疫总局科技项目(2016QK109)
【分类号】：O657.71;TS262.4

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