实时荧光PCR法在鱼翅鉴别中的应用

发布时间：2018-02-12 22:50

本文关键词： 鱼翅实时荧光PCR 灵敏度特异性鉴别　出处：《食品研究与开发》2017年24期 　论文类型：期刊论文

【摘要】：为有效鉴别市售鱼翅的真伪,针对鱼翅中鲨鱼源性成分的线粒体基因组的部分序列,利用实时荧光聚合酶链式反应(Polymerase Chain Reaction,PCR)技术,通过特异性引物和探针的设计建立起一种有效的真伪鉴别方法。该方法中对样品脱氧核糖核酸(Deoxyribonucleic acid,DNA)的提取方法、DNA检测的浓度灵敏度、质量灵敏度以及方法的特异性进行研究。结果表明鱼翅样品用试剂盒进行提取效果良好,其检测的DNA浓度灵敏度可达1×10~(-5) ng/μL,质量灵敏度可达0.01%。利用该方法对市场上的购买的47份鱼翅类样品和18份非鱼翅样品进行了检测,43样品检测出鲨鱼成分,包括4份仿鱼翅在内的22份未检出鲨鱼成分。
[Abstract]:In order to effectively identify the authenticity of shark fin on the market, a real-time fluorescent polymerase chain reaction (PCR) technique was used to detect the mitochondrial genome sequence of shark derived components in shark fin. An effective method for identification of true and false DNA was established by the design of specific primers and probes. In this method, the concentration sensitivity of DNA was determined by the method of DNA extraction from the sample of deoxyribonucleic acid DNA. The quality sensitivity and the specificity of the method were studied. The sensitivity of DNA concentration can reach 1 脳 10 ~ (-5) ng / 渭 L, and the quality sensitivity can reach 0.01. Using this method, the shark composition was detected in 47 shark fin samples and 18 non-shark fin samples. Shark ingredients were not detected in 22 samples, including 4 shark fin imitations.
【作者单位】：中国广州分析测试中心广东省化学危害应急检测技术重点实验室;
【基金】：广东省省级科技计划项目(2013B020501005、2015B090906023)
【分类号】：O657.3;TS254.7

【相似文献】