固态纳米孔对蛋白质易位的实验研究

发布时间：2018-02-16 02:46

本文关键词： 牛血清蛋白(BSA) 抗原抗体固态纳米孔单分子检测　出处：《化学学报》2017年11期 　论文类型：期刊论文

【摘要】：蛋白质因其多样性和功能性,是生物体内一类非常重要的分子.通常蛋白质的表征需要借助荧光或者酶的标记.而纳米孔技术,得益于免标记、单分子检测等优势,为蛋白质的表征提供了新方向.我们使用固态纳米孔完成了单个蛋白质分子及蛋白质-蛋白质结合物的检测.可以发现,外部电压和电解质溶液的酸碱度会直接影响蛋白质分子表面带电量,从而加快或延迟其在孔内的易位时间.抗原、抗体本质上都是蛋白质,两者之间具有高度特异性.通过比较抗体溶液在添加特异性抗原前后的易位事件,实现了单个蛋白质分子和蛋白质-蛋白质结合物的区分.未来,纳米孔技术有望应用于多蛋白质分子的辨识、蛋白质分子相互作用机制等方面的研究.
[Abstract]:Proteins are a very important class of molecules in organisms because of their diversity and functionality. Usually, the characterization of proteins requires fluorescence or enzyme labeling. However, nanopore technology benefits from the advantages of free labeling, single molecule detection, and so on. This provides a new direction for the characterization of proteins. We have completed the detection of single protein molecules and protein-protein conjugates using solid nanoparticles. The external voltage and the pH of the electrolyte solution will directly affect the surface charge of protein molecules, thus accelerating or delaying their translocation time in the pore. By comparing the translocation events of antibody solution before and after adding specific antigen, the distinction between single protein molecule and protein-protein conjugate is realized. Nano-pore technology is expected to be applied to the identification of multi-protein molecules and the mechanism of protein-molecule interaction.
【作者单位】：东南大学江苏省微纳生物医疗器械设计与制造重点实验室;东南大学机械工程学院;南京医科大学口腔疾病研究江苏省重点实验室;南京医科大学附属口腔医院口腔预防科;
【基金】：国家自然科学基金(Nos.51375092,51435003,51675101) 中央高校基本科研业务费专项资金(No.2242015R30002)资助~~
【分类号】：O629.73

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