烟气暴露对大鼠代谢轮廓谱的影响

发布时间：2018-02-20 14:52

本文关键词： 液相色谱-质谱(LC/MS) 代谢组学天然本草添加卷烟血清尿液生物标志物　出处：《质谱学报》2017年04期 　论文类型：期刊论文

【摘要】：运用基于液相色谱-质谱联用技术的代谢组学方法研究了不同卷烟烟气暴露对大鼠内源小分子代谢物组的影响。分别建立了大鼠血清和尿液的代谢轮廓谱,分析了烟气暴露7天、14天和30天时对照组大鼠、普通卷烟暴露组大鼠及含有天然本草添加剂的某品牌卷烟暴露组大鼠的血浆和尿液样本,采用偏最小二乘判别分析(PLS-DA)对数据进行模式识别。结果表明,普通卷烟和天然本草添加卷烟均会影响大鼠整体代谢状态,干扰大鼠磷脂、能量代谢,并对其造成氧化损伤,但天然本草添加卷烟对大鼠的损伤程度低于普通卷烟。在烟气暴露30天时,一些重要标志物在各组相对含量的变化进一步证实了天然本草添加卷烟可降低烟气对大鼠整体代谢的影响,减轻烟气造成的损伤。因此,在烟草中加入天然本草添加剂可在一定程度上减少烟气对机体的伤害,改善体内因烟气干扰而紊乱的磷脂和能量代谢。
[Abstract]:The effects of cigarette smoke exposure on endogenous small molecule metabolites in rats were studied by using the method of metabolomics based on liquid chromatography-mass spectrometry. The metabolic profiles of rat serum and urine were established respectively. The plasma and urine samples of rats in the control group, common cigarette exposure group and a brand cigarette exposed group containing natural herbal additive were analyzed at 7 days, 14 days and 30 days, respectively. Partial least squares discriminant analysis (PLS-DA) was used to recognize the data. The results showed that both common cigarettes and natural herbs could affect the whole metabolic state of rats, interfere with phospholipid and energy metabolism, and cause oxidative damage to them. But the damage of natural herbs added cigarettes to rats was lower than that of common cigarettes. After 30 days of smoke exposure, The changes of relative contents of some important markers in each group further confirmed that natural herbs added cigarettes can reduce the effect of smoke on the overall metabolism of rats and alleviate the damage caused by smoke. Adding natural herbal additive to tobacco can reduce the harm of smoke to the body to a certain extent and improve the phospholipid and energy metabolism which is disturbed by smoke disturbance in the body.
【作者单位】：珠海清大弘瑞生物科技有限公司;清华大学化学系;江西中烟工业有限责任公司;
【基金】：中国烟草总公司重大专项(110201401025(JH-03))资助
【分类号】：O657.63;R114

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