SERS光谱研究细胞色素在过渡金属表面的电子转移

发布时间：2018-02-27 16:08

本文关键词： 细胞色素 SERS 过渡金属电子转移生物相容性　出处：《吉林大学》2017年硕士论文　论文类型：学位论文

【摘要】：细胞色素是生物体内一类重要的血红素蛋白,广泛参与细胞的能量转移、新陈代谢、细胞凋亡等生物反应过程。体外研究细胞色素的结构和功能对于药物代谢、临床医学、肿瘤生化等领域具有重大的研究意义。本论文利用过渡金属作为活性界面,通过表面增强拉曼散射(SERS)光谱研究细胞色素的电子转移。主要创新点如下:(1)首次利用过渡金属镍岛膜作为活性基底,利用SERS光谱研究细胞色素b_5与镍之间的电子转移。结果表明,氧化态细胞色素b_5能够从镍表面接收电子变成还原态,而且蛋白质没有变性,证明镍具有良好的生物相容性。(2)以镍岛膜为界面,研究了细胞色素b_5与肌红蛋白之间的电子传递。通过时间依赖的SERS光谱观察到了二者之间的电子转移过程,从而进一步证实了细胞色素b_5在镍基底表面保持了其生物活性。(3)对镍基底表面进行了粗糙化处理,探究了表面形态对细胞色素c拉曼强度的影响,并对增强因子进行了估算。(4)制备了过渡金属纳米材料(镍纳米线和钴纳米粒子),首次发现了二者对细胞色素c的还原活性。(5)制备了金、银贵金属纳米粒子,对比了过渡金属及贵金属纳米粒子与细胞色素c之间电子转移方式、生物相容性及拉曼增强能力,并对其电子转移机理提出了合理解释。
[Abstract]:Cytochrome is a kind of important heme protein in organism. It is widely involved in the biological reaction process such as energy transfer, metabolism, apoptosis and so on. In vitro, the structure and function of cytochrome on drug metabolism, clinical medicine, and so on are studied. In this paper, transition metals are used as the active interface. Electron transfer of cytochrome was studied by surface-enhanced Raman scattering spectroscopy (SERS). The main innovations are as follows: 1) for the first time, the transition metal nickel island film is used as the active substrate. The electron transfer between cytochrome B5 and nickel was studied by SERS spectroscopy. The results show that oxidized cytochrome B5 can receive electrons from nickel surface into reduced state, and the protein has no denaturation. It was proved that nickel had good biocompatibility. (2) the electron transfer between cytochrome b5 and myoglobin was studied using nickel island membrane as the interface. The electron transfer process between them was observed by time-dependent SERS spectroscopy. It is further confirmed that cytochrome B5 maintains its biological activity on the surface of nickel substrate, and that the surface of nickel substrate is roughened, and the effect of surface morphology on the Raman intensity of cytochrome c is investigated. The transition metal nanomaterials (nickel nanowires and cobalt nanocrystals) were prepared by estimating the enhancement factor. Gold and silver noble metal nanoparticles were first found to be active in cytochrome c reduction. The electron transfer mode biocompatibility and Raman enhancement ability between transition metal and precious metal nanoparticles and cytochrome c were compared and the mechanism of electron transfer was explained.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：Q51;O657.37

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