表面活性剂对T1脂肪酶活力的影响

发布时间：2018-02-28 18:32

本文关键词： T脂肪酶表面活性剂临界胶束浓度激活　出处：《现代食品科技》2017年03期 　论文类型：期刊论文

【摘要】：本文研究了非离子型、阳离子型、阴离子型以及两性离子型表面活性剂对T1脂肪酶活力的影响。采用不同浓度的表面活性剂对T1脂肪酶进行处理,再以对硝基苯酚月桂酸酯作为底物,测定处理后的酶活变化。研究发现,低浓度的非离子型表面活性剂对T1都有激活作用,使其相对酶活提高50%到150%。当非离子型表面活性剂的浓度超过其临界胶束浓度,T1的活力受到不同程度的抑制,其中吐温80的抑制作用最为明显。阳离子型(CTAB)、阴离子型(N-L与SDS)和两性离子型(SB3-14)表面活性剂都对脂肪酶的酶活有强烈的抑制作用,可能是因为离子型表面活性剂的带电基团与蛋白质表面的带电氨基酸产生强烈的电荷相斥作用,导致蛋白变性而失去活力。T1脂肪酶是一类具有工业应用价值的耐热脂肪酶,研究不同类型的表面活性剂对其活力的影响,对于在不同目的下选择使用适合的表面活性剂具有重要意义。
[Abstract]:The effects of Nonionic, cationic, anionic and amphoteric surfactants on the activity of T1 lipase were studied. The enzyme activity after treatment was determined by using p-nitrophenol lauric acid ester as substrate. It was found that low concentration of Nonionic surfactants could activate T1. When the concentration of Nonionic surfactants exceeds its critical micelle concentration, the activity of T 1 is inhibited to varying degrees, and its relative enzyme activity is increased by 50% to 1500.When the concentration of Nonionic surfactants exceeds its critical micelle concentration, The inhibitory effect of Tween 80 on lipase activity was most obvious. Cationic CTABN, anionic N-L and SDS) and amphoteric type SB3-14) surfactants all had strong inhibitory effects on lipase activity. It may be that the charged groups of ionic surfactants have a strong charge repulsive effect with the charged amino acids on the surface of proteins, resulting in the denaturation of proteins and the loss of activity. T1 lipases are a class of thermostable lipases of industrial value. It is of great significance to study the effect of different surfactants on their vitality for the purpose of choosing suitable surfactants for different purposes.
【作者单位】：华南理工大学食品科学与工程学院;
【基金】：国家自然科学基金资助项目(21406076) 国家高技术研究发展计划(863项目)(2014AA091701)
【分类号】：O643.36;O647.2

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