溶酶体荧光探针研究新进展

发布时间：2018-03-02 21:18

本文选题：溶酶体　切入点：荧光探针　出处：《有机化学》2017年02期 　论文类型：期刊论文

【摘要】：溶酶体在细胞各种生命活动,如物质代谢、细胞膜循环、细胞凋亡中发挥着重要作用.将溶酶体可视化,并对其活性物种、特定微环境及关键生理过程进行检测,不仅有助于理解溶酶体参与生命活动的分子机制,而且对疾病的治疗具有重要的指导意义.近年来,许多靶向溶酶体的荧光探针被相继报道,其检测对象包括还原性物种、活性氧、活性氮、氢离子、金属离子、阴离子以及生物酶等,还包括对温度、粘度、极性、p H等微环境的检测.溶酶体的荧光探针的定位机制分为三类:利用溶酶体的酸性生理环境、利用溶酶体的物质代谢功能以及利用溶酶体特殊的膜蛋白或水解酶.对近年来出现的主要溶酶体荧光探针进行了总结与评述,展望了溶酶体荧光探针应用于探究重要细胞生理过程的分子机制,以及相关疾病诊断方面的发展趋势.
[Abstract]:Lysosomes play an important role in various cell life activities, such as substance metabolism, cell membrane circulation and apoptosis. The lysosomes are visualized and detected for their active species, specific microenvironments and key physiological processes. Not only is it helpful to understand the molecular mechanism of lysosome's involvement in life, but it is also of great significance in the treatment of diseases. In recent years, a number of fluorescent probes targeting lysosomes have been reported, including the detection of reductive species. Reactive oxygen species, active nitrogen, hydrogen ions, metal ions, anions, biological enzymes, and so on, including temperature, viscosity, The localization mechanism of lysosomal fluorescence probe is divided into three categories: using the acidic physiological environment of lysosome, Based on the metabolic function of lysosome and the special membrane protein or hydrolase of lysosome, the main lysosomal fluorescent probes in recent years were summarized and reviewed. The application of lysosomal fluorescence probe to the molecular mechanism of important cellular physiological processes and the development trend of diagnosis of related diseases are prospected.
【作者单位】：北京师范大学化学学院;
【基金】：国家自然科学基金(No.21272027)资助项目~~
【分类号】：O657.3

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