GC-MS研究含天然麝香小金丸与含人工麝香小金丸特征化学成分

发布时间：2018-03-03 19:02

本文选题：小金丸　切入点：气相色谱-质谱　出处：《中国实验方剂学杂志》2017年02期 　论文类型：期刊论文

【摘要】：目的:建立GC-MS法分析小金丸中的特征成分。方法:采用无水乙醇超声提取小金丸中的挥发性成分,再采用气相色谱-质谱联用技术和自动质谱解卷积定性系统(AMDIS)对其特征成分进行定性分析。结果:共鉴定出53个化学成分,含天然麝香小金丸中鉴定出30个化合物,而含人工麝香小金丸中鉴定出23个化合物。含天然麝香小金丸中检测出7个特征成分,分别是棕榈酸乙酯;乙酸脱氢异雄甾酮;3-乙基-3-羟基-雄甾烷-17-酮;去氢表雄酮;雄甾酮;3,17-二酮雄甾烷;3,17-二醇雄甾烷,其相对质量分数分别为0.11%,1.12%,2.80%,1.59%,0.56%,1.99%,1.22%。特征成分中的甾体类化合物来自于天然麝香,酯类化合物可能是天然麝香中的某些化合物与其他药物配伍反应生成的物质。结论:该方法特征性强、方法简便、重复性好,为区分含天然麝香小金丸和含人工麝香小金丸提供参考依据。
[Abstract]:Objective: to establish a GC-MS method for the analysis of the characteristic components in Xiaojin pills. Gas chromatography-mass spectrometry (GC-MS) and automatic mass spectrometry deconvolution system (AMDIS) were used to qualitatively analyze the characteristic components. Results: a total of 53 chemical constituents were identified, including 30 compounds in natural Musk Xiaojin pills. And 23 compounds were identified in Shixiaojin pills containing artificial musk, 7 characteristic components were detected in natural Musk Xiaojin pills, which were ethyl palmitate, 3-ethyl-3-hydroxy-androstane-17 ketone, dehydroepiandrosterone, dehydroepiandrosterone, dehydroepiandrosterone, dehydroepiandrosterone, dehydroepiandrosterone, dehydroepiandrosterone and dehydroepiandrosterone respectively. The relative mass fraction of androsterane is 0.11 and the relative mass fraction is 1.122.80 and 1.590.560.991.22.The steroids in the characteristic components are derived from natural musk. The ester compounds may be formed by the compatibility of some compounds in natural musk with other drugs. Conclusion: this method is characterized by strong characteristics, simple and reproducible. It provides reference for distinguishing natural Musk Xiaojin pills from artificial Musk Xiaojin pills.
【作者单位】：西南民族大学药学院;
【基金】：四川省科技厅科技支撑项目(2015SZ0040) 西南民族大学研究生创新科研项目(CX2015SZ044)
【分类号】：R286.0;O657.63

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