热诱导白蛋白与壳聚糖在溶液中的自组装

发布时间：2018-03-05 07:47

本文选题：热诱导　切入点：蛋白质　出处：《高分子学报》2016年01期 　论文类型：期刊论文

【摘要】：80℃热诱导牛血清白蛋白(BSA)变性,BSA疏水残基暴露在分子表面,并进一步凝胶化.采用紫外-可见光光度仪(UV-Vis)和动态激光光散射仪(DLS)考察了热处理时间、溶液p H值、壳聚糖(CS)与BSA配比等因素对CS与BSA自组装行为的影响.加热时间介于15~20 min,BSA与CS发生自组装;BSA/CS混合体系p H大于BSA等电点(p I=4.7)时,BSA与壳聚糖静电作用增强,随着p H增大,出现沉淀.BSA浓度过高,变性BSA之间聚集作用增强,导致体系出现沉淀.研究结果显示,CS阻碍了BSA自身凝胶化进程,并与BSA发生自组装.在p H=5.4的溶液中制备了BSA-CS自组装体.采用透射电镜(TEM)观察了自组装体的形态,采用DLS对其粒径及其分布进行了测试.自组装体是以BSA聚集体为核、CS分子链为壳的核壳纳米结构.制备的自组装体室温贮存40天后,其粒径和分布无明显变化.
[Abstract]:Induced by bovine serum albumin (BSA) 80 thermal denaturation, BSA hydrophobic residues exposed on the molecular surface, and further gelation. The UV visible light spectrometer (UV-Vis) and dynamic laser light scattering (DLS) heat treatment time was investigated, the P solution H value, chitosan (CS) and the BSA ratio of CS and BSA. The self-assembly behavior of the heating time between 15~20 min, BSA and CS self assembly; hybrid BSA/CS system p H is greater than the isoelectric point of BSA (P I=4.7), enhance the effect of BSA and chitosan with P H electrostatic precipitation increases,.BSA concentration between variable BSA aggregation enhanced system leads to precipitation. The results of the study showed that CS inhibited the BSA its gelation process, and self-assembly in solution with BSA. P H=5.4 in preparation of BSA-CS self-assembly by transmission electron microscopy (TEM) observation of the self-assembly morphology, using DLS on the particle the size and distribution of The self-assembly is a core shell nanostructure with BSA aggregate as core and CS molecular chain as shell. The self-assembled assembly has no obvious change in particle size and distribution after 40 days of storage at room temperature.

【作者单位】：西北师范大学化学化工学院生态环境相关高分子材料教育部重点实验室;兰州交通大学化学与生物工程学院;
【基金】：国家自然科学基金(基金号21164003,21263024,21364012) 甘肃省自然科学基金(基金号1308RJZA13)资助
【分类号】：O641.3

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