竞争法免疫层析试条的数学模型与仿真研究

发布时间：2018-03-05 20:00

本文选题：免疫层析试条　切入点：竞争法　出处：《分析化学》2017年09期 　论文类型：期刊论文

【摘要】：根据竞争法免疫层析试条的检测原理,本研究将其分为TwA和TnA两种模式,结合对流扩散方程分别建立其数学模型,并运用COMSOL软件对试条的动态反应过程进行仿真,得到检测线和质控线上各复合物浓度关于各影响因素的关系曲线。在TwA模式中,分析了待测物浓度[A_0]=0~20 mol/L,标记物浓度[P_0]=0.01~100 mol/L,检测线位于5~20 mm位置等因素对于检测结果的影响;在TnA模式中,分析了[A_0]=0~20 mol/L,[P_0]=0.01~100 mol/L,[A_0]和[P_0]两种物质浓度及孔隙率等因素对于检测结果的影响。结果表明,本研究建立的竞争法模型与仿真能探究各参数对于检测结果的影响,优化试条性能,从而提高试条检测灵敏度和实现定量检测。
[Abstract]:According to the detection principle of competition law immunochromatographic strip, this study divides it into two kinds of models: TwA and TnA. Combined with convection-diffusion equation, the mathematical model is established, and the dynamic reaction process of the strip is simulated by COMSOL software. The relationship curves of the concentration of each complex between the detection line and the quality control line were obtained. In the TwA model, the effects of the concentration of the substance to be tested [A 0] 0 / L, the concentration of labeled substance [P0] 0.01 ~ 100mol / L, and the location of the detection line at 520 mm were analyzed. In the TnA model, the effects of the concentration and porosity of two kinds of substances, such as the concentration and porosity of two substances, [Astu0] / 20 mol / L, [P0] 0.01mol / L, [P0] 0.01mol / L, [Astu0] and [P0], on the test results are analyzed. The results show that the competition law model and simulation established in this study can explore the influence of the parameters on the test results. The performance of test strip is optimized to improve the sensitivity of test strip and to realize quantitative detection.
【作者单位】：厦门大学仪器与电气系;福州大学福建省医疗器械和医药技术重点实验室;
【基金】：中国工程院与英国皇家工程院中英校企合作项目(No.UK-CIAPP\276) 国家自然科学基金(No.61403319) 福建省自然科学基金(No.2015J05131) 福建省生态产业绿色技术重点实验室开放课题项目资助~~
【分类号】：O658.1

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