QuEChERS-超高效液相色谱-串联质谱法快速检测鸡肉中8种常见兽药残留

发布时间：2018-03-11 13:30

本文选题：QuEChERS　切入点：超高效液相色谱-串联三重四级杆质谱　出处：《卫生研究》2017年01期 　论文类型：期刊论文

【摘要】：目的建立Qu ECh ERS-超高效液相色谱-串联三重四级杆质谱法(UPLCMS/MS)测定鸡肉基质中3类8种兽药残留的检测技术。方法鸡肉样品采用酸化乙腈提取,盐析后取乙腈相,用十八烷基硅烷(C18)和PSA吸附剂分散固相萃取净化,采用Waters ACQUITYTM BEH C18色谱柱(100 mm×2.1 mm×1.7μm)分离后于电喷雾正离子源(ESI+)和多反应监测(MRM)模式下进行测定,基质标准曲线结合稳定同位素内标法定量。结果 8种目标化合物阴性加标水平在1、2和4μg/kg下,回收率为81.2%~94.1%,RSD在4.2%~14.3%。8种目标化合物的检出限在0.1~0.2μg/kg,定量限在0.2~0.5μg/kg。对30件鸡肉样品进行目标化合物的检测,未检测到超限量的兽药残留。结论 UPLC-MS/MS法操作简便、快速,灵敏度和准确度高,包含种类较宽,具有拓展性,适用于鸡肉中该类兽药残留的日常监测。
[Abstract]:Objective to establish a Qu ECh ERS- ultra performance liquid chromatography tandem mass spectrometry three level Four rod (UPLCMS/MS) determination of matrix of 3 kinds of chicken 8 kinds of veterinary drug residues detection method. Samples were acidified acetonitrile extraction, salting out after the acetonitrile phase, with eighteen alkyl silane (C18) and PSA dispersive solid phase adsorbent using Waters ACQUITYTM BEH extraction, C18 column (100 mm * 2.1 mm * 1.7 m) in the positive electrospray ion source separation (ESI+) and multiple reaction monitoring (MRM) mode were determined. The standard curve matrix combined with stable isotope internal standard method. The results of the 8 target compounds negative spiked levels in the 1,2 and 4 g/kg, the recovery rate was 81.2%~94.1%, RSD in 0.1~0.2 g/kg 4.2%~14.3%.8 in the detection limits of target compounds, the quantitative detection limit of target compounds of 30 chicken samples in 0.2~0.5 g/kg., was not detected in veterinary drug residues. Conclusion the ultra Limited The UPLC-MS/MS method is easy to operate, fast, sensitive and accurate, including wide variety and expansibility. It is suitable for daily monitoring of this kind of veterinary drug residues in chicken.

【作者单位】：北京市疾病预防控制中心;
【基金】：国家自然科学基金(No.81302431)
【分类号】：TS251.7;O657.63

【参考文献】