蜂蜜中原蜜黄酮类化合物HPLC图谱比较分析

发布时间：2018-03-19 00:28

本文选题：油菜原蜜　切入点：高效液相色谱　出处：《食品科学》2017年14期 　论文类型：期刊论文

【摘要】：利用高效液相色谱比较分析不同蜂种、不同产地、加工与否以及不同蜜源蜂蜜中原蜜黄酮类化合物的种类及含量差异。结果表明,意大利蜜蜂油菜原蜜和中华蜜蜂油菜原蜜总峰数和峰形都比较相似,意大利蜜蜂油菜原蜜的总黄酮含量比中华蜜蜂油菜原蜜总黄酮含量高。不同产地的意大利蜜蜂油菜原蜜的图谱峰形整体相似,总黄酮含量和黄酮类化合物种类大致相同。加工后的商品蜜总黄酮含量减少,黄酮类化合物的种类也减少,商品蜜的高效液相色谱图显示其黄酮类化合物的出峰时间靠前,主要密集分布在水溶性的分离相中。3个不同蜜源蜂蜜原蜜黄酮类化合物高效液相色谱图的总峰数和峰形都有较大差异,油菜原蜜、洋槐原蜜及柑橘原蜜的总峰数分别为65、58、70个,柑橘原蜜的总黄酮含量最高,达161.62μg/100 g。单花蜂蜜原蜜黄酮类化合物的高效液相色谱图峰形具有一定的特异性,可以作为指纹图谱用于蜂蜜蜜源的鉴定。
[Abstract]:The species and contents of flavonoids in honey from different honeybee species, different producing areas, processed honey and honey honey were compared and analyzed by high performance liquid chromatography (HPLC). The total peak number and shape of the original honey of Italian bee rape and Chinese bee rape were similar. The total flavonoids content of the original honey of Italian bee rape was higher than that of the Chinese bee rape. The content of total flavonoids is approximately the same as the kinds of flavonoids. After processing, the total flavonoids content of commercial honey is reduced, and the kinds of flavonoids are also reduced. The high performance liquid chromatography of commercial honey shows that the peak time of flavonoids is ahead. The total peak number and peak shape of the three honey flavonoids from different honey sources were significantly different. The total peak numbers of rape honey, locust honey and citrus honey were 65.58 and 70, respectively. The content of total flavonoids in citrus honey was the highest, reaching 161.62 渭 g / 100 g. The peak shape of the flavonoids in honey from single flower honey had certain specificity, which could be used as fingerprint to identify honey honey source.
【作者单位】：重庆师范大学生命科学学院;
【基金】：重庆市社会民生科技创新专项(cstc2015shmszx80031) 教育部留学回国人员科研启动基金项目
【分类号】：O657.72;S896.1

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