基于水溶性共轭聚合物PPE-OBS的荧光化学传感器的制备及应用研究

发布时间：2018-03-20 03:28

本文选题：水溶性共轭聚合物　切入点：汞离子　出处：《吉林大学》2017年硕士论文　论文类型：学位论文

【摘要】：水溶性共轭聚合物是指主链含有较大的π-π*共轭电子结构,侧链上修饰了亲水基团的一类线性高分子化合物。它不但具有较高的量子产率、稳定的光学性质、显著的荧光放大现象和超猝灭效应,而且还具有良好的水溶性和较强的静电作用。基于上述优点,水溶性共轭聚合物已经被广泛地用作荧光生物化学传感器,实现了对金属离子、生物分子和有机小分子等的高灵敏检测。在此基础上,我们制备了一种羧基修饰的水溶性共轭聚合物聚(2,5-双(4-氧基丁酸钠)-1,4-苯基亚乙炔基-1,4-亚苯基亚乙炔基(简称PPE-OBS),并研究了该聚合物在金属离子和半胱氨酸类生物分子传感方面的应用。第一章,对共轭聚合物进行概述,介绍了共轭聚合物的分类以及水溶性共轭聚合物在传感金属离子、生物分子、有机小分子等领域的应用现状。第二章,详细地说明了水溶性共轭聚合物PPE-OBS的合成过程,并利用核磁共振仪、质谱仪、红外光谱仪、热重分析仪和凝胶渗透色谱仪对中间产物和目标产物进行了表征。第三章,设计了一种基于PPE-OBS的Hg~(2+)荧光传感器。我们首先对实验条件进行了优化,并借助荧光显微镜探讨了该荧光传感器的传感机制。研究表明,在最佳实验条件下,该传感器检测Hg~(2+)的线性范围为6×10-8~8×10-5 mol L-1,检出限低至2.10×10-9 mol L-1。最后,我们将该传感器应用于环境水样中Hg~(2+)的检测。第四章,基于Hg~(2+)和PPE-OBS形成的复合体系,我们成功地研制了一种基于荧光turn-on现象的半胱氨酸类生物分子的荧光传感器。在最优的实验条件下,该传感器相比于其他传感器具有良好的线性关系,较高的灵敏度和选择性。该传感器对半胱氨酸(Cys)、同型半胱氨酸(Hcy)和还原型谷胱甘肽(GSH)的检测下限分别为0.725μmol L-1,0.982μmol L-1和1.21μmol L-1。最后,我们将该传感器应用于绿茶饮料和蜂蜜样品中的Cys含量的检测,并得到了满意的实验结果,证明了该方法具有实用性和可靠性。
[Abstract]:The water-soluble conjugated polymer is a kind of linear polymer with large 蟺-蟺 * conjugated electronic structure in the main chain and modified hydrophilic group on the side chain. It has high quantum yield and stable optical properties. Because of the remarkable fluorescence amplification and hyperquenching effect, the water-soluble conjugated polymer has been widely used as a fluorescence biochemistry sensor because of its good water-solubility and strong electrostatic effect. The highly sensitive detection of metal ions, biomolecules and organic small molecules has been realized. We have prepared a carboxyl modified water-soluble conjugated polymer, poly (2) -5- bis (4-oxy) -4-oxy (4-phenylacetylethynyl) (PPE-OBS-), and have studied its biofractions in metal ions and cysteine. Applications in subsensing. Chapter 1. The classification of conjugated polymers and the application of water-soluble conjugated polymers in sensing metal ions, biomolecules and organic small molecules are reviewed. The synthesis process of water-soluble conjugated polymer PPE-OBS was described in detail, and the intermediate and target products were characterized by NMR, mass spectrometer, infrared spectrometer, thermogravimetric analyzer and gel permeation chromatography. A Hg~(2) fluorescence sensor based on PPE-OBS is designed. Firstly, the experimental conditions are optimized, and the sensing mechanism of the fluorescence sensor is discussed by means of fluorescence microscope. The linear range of the sensor is 6 脳 10 ~ (-8) ~ 8 脳 10 ~ (-5) mol L ~ (-1) and the detection limit is as low as 2.10 脳 10 ~ (-9) mol L ~ (-1). Finally, we apply the sensor to the detection of Hg~(2 in environmental water samples. Chapter 4th, based on the composite system of Hg~(2) and PPE-OBS, We have successfully developed a fluorescence sensor for cysteine biomolecules based on fluorescence turn-on phenomenon. Under the optimal experimental conditions, the sensor has a good linear relationship with other sensors. The detection limits of the sensor for cysteine cystatin, homocysteine and reduced glutathione were 0.725 渭 mol L -1 0.92 渭 mol L -1 and 1.21 渭 mol L -1, respectively. The sensor has been applied to the determination of Cys in green tea drinks and honey samples. The satisfactory experimental results show that the method is practical and reliable.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O657.3

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