超声萃取高效液相色谱法测定诺丽果粉中的莨菪亭、芦丁和槲皮素含量

发布时间：2018-03-20 20:33

  本文选题：超声萃取　切入点：莨菪亭　出处：《食品科学》2017年06期 　论文类型：期刊论文

【摘要】：采用超声萃取高效液相色谱法测定诺丽果粉中的莨菪亭、芦丁和槲皮素含量。为了提高萃取效率,实验系统地优化了影响萃取效率的因素(有机溶剂种类、有机溶剂与水的比例、萃取时间、萃取温度和超声频率)。结果表明,最佳萃取条件:萃取剂为体积分数80%甲醇溶液,萃取时间为30 min,萃取温度为20℃,超声频率为45 k Hz。采用依利特Hypersil ODS柱(4.6 mm×250 mm,5μm),以甲醇-0.1%三氟乙酸-乙腈溶液作为流动相进行梯度淋洗,流速1.0 m L/min,在检测波长365 nm,柱温25℃条件下,对莨菪亭、芦丁和槲皮素分离检测。方法具有良好的线性关系(r2≥0.999 8)、检出限(≤1.30μg/L)、回收率(90.67%~113.00%)和重复性(相对标准偏差不大于1.46%,n=8)。该方法准确可靠,可用于定性、定量测定诺丽果粉样品中的莨菪亭、芦丁和槲皮素。
[Abstract]:The contents of scopoletin, rutin and quercetin in Nori fruit powder were determined by ultrasonic extraction high performance liquid chromatography. In order to improve the extraction efficiency, the factors affecting the extraction efficiency were systematically optimized. The ratio of organic solvent to water, extraction time, extraction temperature and ultrasonic frequency are determined. The results show that the optimum extraction conditions are as follows: volume fraction 80% methanol solution, extraction time 30 min, extraction temperature 20 鈩，

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