二醇基硅胶固相萃取-多维质谱对乌鳢组织脂质组学分析

发布时间：2018-03-22 12:21

本文选题：鸟枪法脂质组学　切入点：多维质谱　出处：《食品科学》2017年02期 　论文类型：期刊论文

【摘要】：建立以二醇基键合硅胶(Si O2/diol)为填料的固相萃取(solid-phase extraction,SPE)和基于多维度串联质谱的鸟枪法分离纯化并测定乌鳢肌肉组织中的磷脂。利用Folch法和SPE法提取并纯化磷脂,比较3种不同填料SPE柱的萃取效果,优化SPE参数,最佳条件:上样量100?μL,淋洗液p H值为2,洗脱液为体积分数50%乙腈溶液1 m L。所得提取物以直接进样的方法流动注射入电喷雾离子源,经三重四极杆质谱的母离子扫描和中性丢失扫描模式,实现对磷脂分子种类的鉴定和定量。同时,通过多维度串联质谱分析,得到各个磷脂分子中2条脂肪酸链长度和不饱和度信息。实验结果显示,乌鳢样品中成功检出磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰肌醇和磷脂酰丝氨酸4类磷脂,共计67种磷脂分子,且大部分磷脂均含有不饱和脂肪酸链。本方法简单高效,分析准确快捷,结果稳定。
[Abstract]:Solid phase extraction (SPE) with diol bonded silica gel (Sio _ 2 / diol) as filler and bird gun method based on multi-dimensional tandem mass spectrometry were used to isolate and determine phospholipid in the muscle tissue of snakehead Argus Argus. Phospholipid was extracted and purified by Folch and SPE. The extraction efficiency of three different packing SPE columns was compared and the SPE parameters were optimized. The optimum conditions were as follows: sample amount 100? 渭 L, pH value of eluant was 2, elution solution was 50% acetonitrile solution 1 mL by volume fraction. The extract was injected into electrospray ion source by direct injection method, and was scanned by mother ion and neutral loss by triple quadrupole mass spectrometry. The identification and quantification of phospholipids were realized. At the same time, the length and unsaturation information of two fatty acid chains in each phospholipid molecule were obtained by multi-dimensional tandem mass spectrometry. Phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and phosphatidyl serine were successfully detected in snakehead samples. The analysis is accurate and quick, and the result is stable.
【作者单位】：浙江工商大学海洋食品研究院;
【基金】：国家国际科技合作专项(2014DFA32880) 国家自然科学基金青年科学基金项目(31601542) 浙江省自然科学基金项目(LQ16C200001)
【分类号】：TS254.7;O657.63

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