超高效液相色谱-串联质谱法检测鸡血浆中头孢噻肟及其主要代谢物

发布时间：2018-03-23 04:39

本文选题：头孢噻肟(CTX)　切入点：去乙酰头孢噻肟(DCTX)　出处：《食品科学》2017年22期 　论文类型：期刊论文

【摘要】：研究头孢噻肟(cefotaxime,CTX)在鸡血浆中代谢规律,建立同时检测血浆中CTX及其主要代谢物去乙酰头孢噻肟(desacetylcefotaxime,DCTX)的超高效液相色谱-串联质谱方法,以10 mg/kg的体质量剂量给药实验蛋鸡,并对给药后不同时间点采集的血液样品,进行CTX及其主要代谢物DCTX含量的检测。结果表明,所建方法中CTX和DCTX的检出限分别为0.07、0.14μg/L;定量限分别为0.23、0.99μg/L。回收率分别为86.7%~95.2%、89.5%~112%,相对标准偏差不大于7.7%,满足残留分析方法的要求;CTX在蛋鸡体内分布迅速,给药后即刻达到峰值(56.34 mg/L),且代谢较快,给药24 h后检测值低于3.0μg/L,而主要代谢物DCTX在给药45 min后达到峰值(10.22 mg/L),消除相对缓慢,给药24 h时检测值仍可达到54.0μg/L。
[Abstract]:To study the metabolic characteristics of cefotaxime (CTX) in chicken plasma, and to establish a high performance liquid chromatography-tandem mass spectrometry method for simultaneous determination of CTX and its main metabolite, desacetylcefotaxime, in chicken plasma. The contents of CTX and its main metabolite DCTX in blood samples collected at different time points after administration were determined. The detection limits of CTX and DCTX were 0.07 ~ 0.14 渭 g / L and 0.23 ~ 0.99 渭 g / L, respectively. The recoveries of CTX and DCTX were 86.7% and 95.2 渭 g / L, respectively. The relative standard deviation was not more than 7.70.CTX distributed rapidly in layers. The peak value (56.34 mg / L) was reached immediately after administration, and the metabolic rate was faster. The detection value of DCTX was lower than 3.0 渭 g / L 24 h after administration, while the peak value of DCTX reached 10.22 mg / L after 45 min administration, and the elimination was relatively slow. The detection value was still up to 54.0 渭 g / L at 24 h.
【作者单位】：南京农业大学食品科技学院肉品加工与质量控制教育部重点实验室;中国农业科学院农业质量标准与检测技术研究所农业部农产品质量安全重点实验室;
【基金】：中国农业科学院科技创新工程项目
【分类号】：O657.63;TS251.7

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